Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery.

Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Research Abstract Details 

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Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Abstract Text:

Q Kang,M H Sun,H Cheng,Y Peng,A G Montag,A T Deyrup,W Jiang,H H Luu,J Luo,J P Szatkowski,P Vanichakarn,J Y Park,Y Li,R C Haydon,T-C He,Q Kang,M H Sun,H Cheng,Y Peng,A G Montag,A T Deyrup,W Jiang,H H Luu,J Luo,J P Szatkowski,P Vanichakarn,J Y Park,Y Li,R C Haydon,T-C He,

Efficacious bone regeneration could revolutionize the clinical management of bone and musculoskeletal disorders. Although several bone morphogenetic proteins (BMPs) (mostly BMP-2 and BMP-7) have been shown to induce bone formation, it is unclear whether the currently used BMPs represent the most osteogenic ones. Until recently, comprehensive analysis of osteogenic activity of all BMPs has been hampered by the fact that recombinant proteins are either not biologically active or not available for all BMPs. In this study, we used recombinant adenoviruses expressing the 14 types of BMPs (AdBMPs), and demonstrated that, in addition to currently used BMP-2 and BMP-7, BMP-6 and BMP-9 effectively induced orthotopic ossification when either AdBMP-transduced osteoblast progenitors or the viral vectors were injected into the quadriceps of athymic mice. Radiographic and histological evaluation demonstrated that BMP-6 and BMP-9 induced the most robust and mature ossification at multiple time points. BMP-3, a negative regulator of bone formation, was shown to effectively inhibit orthotopic ossification induced by BMP-2, BMP-6, and BMP-7. However, BMP-3 exerted no inhibitory effect on BMP-9-induced bone formation, suggesting that BMP-9 may transduce osteogenic signaling differently. Our findings suggest that BMP-6 and BMP-9 may represent more effective osteogenic factors for bone regeneration.

Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Publishing Authors By Initials

Q Kang,MH Sun,H Cheng,Y Peng,AG Montag,AT Deyrup,W Jiang,HH Luu,J Luo,JP Szatkowski,P Vanichakarn,JY Park,Y Li,RC Haydon,TC He,Q Kang,MH Sun,H Cheng,Y Peng,AG Montag,AT Deyrup,W Jiang,HH Luu,J Luo,JP Szatkowski,P Vanichakarn,JY Park,Y Li,RC Haydon,TC He,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Gene therapy

VOLUME: 11

Page Numbers: 1312-20

Journal Abbreviation: Gene Ther.

ISSN: 0969-7128

DAY: 19

MONTH: Sep

YEAR: 2004

Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9421525

Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: genetics

Chemical & Substance for Abstract: Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery. Information

Substance Name: bone morphogenetic protein 9

Registry Number: 0

Grant and Affiliation Information for Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery.

AFFILIATION: Molecular Oncology Laboratory, Department of Surgery, The University of Chicago Medical Center, Chicago, IL 60637, USA.

Country: England

AGENCY: United States NCCAM

GRANT: F05 AT002014-01

ACRONYM: AT

MEDLINETA: Gene Ther

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