Characterization of the development of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells during osteoinduction by rhBMP-2: a histochemical and ultrastructural study.

Characterization of the development of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells during osteoinduction by rhBMP-2: a histochemical and ultrastructural study. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of the development of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells during osteoinduction by rhBMP-2: a histochemical and ultrastructural study. Abstract Text:

T Nakagawa,T Sugiyama,K Shimizu,T Murata,M Narita,S Nakamura,T Tagawa,

OBJECTIVE: To investigate the characteristics of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells induced by recombinant human bone morphogenetic protein-2 (rhBMP-2). MATERIALS AND METHODS: rhBMP-2 (5 microg) combined with atelocollagen was implanted into calf muscles of rats and removed on days 7, 10, 14, 21, or 28. Tissue sections were examined using: (i) hematoxylin/Alcian blue/Sirius red stain, (ii) enzyme histochemistry for alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase activity, (iii) immunohistochemistry for types I, II, and X collagen, and (iv) electron microscopy. RESULTS: On day 7, numerous fibroblast-like cells with ALP activity were present on the pellet rim. On day 10, chondroid matrix (CM) had formed, contained both type I collagen and proteoglycans, and often continued into the BMP pellet. On day 14, bone-like matrix formed around hypertrophic chondrocytes simultaneously with endochondral ossification. Coexpression of types I and II collagen within chondrocytes and osteocytes was observed throughout the time course of the experiment. CONCLUSION: These results suggest that fibroblast-like cells invading the pellet differentiate into chondrocytes and form CM under the scaffold of the carrier component. It appears that some chondrocytes change their phenotype to produce the bone-like matrix and remain within the endochondral bone. This process enables rapid osteogenesis to occur.

Characterization of the development of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells during osteoinduction by rhBMP-2: a histochemical and ultrastructural study. Publishing Authors By Initials

T Nakagawa,T Sugiyama,K Shimizu,T Murata,M Narita,S Nakamura,T Tagawa,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

Characterization of the development of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells during osteoinduction by rhBMP-2: a histochemical and ultrastructural study. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Oral diseases

VOLUME: 9

Page Numbers: 255-63

Journal Abbreviation:

ISSN: 1354-523X

DAY: 19

MONTH: Sep

YEAR: 2003

Characterization of the development of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells during osteoinduction by rhBMP-2: a histochemical and ultrastructural study. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9508565

Characterization of the development of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells during osteoinduction by rhBMP-2: a histochemical and ultrastructural study. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Characterization of the development of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells during osteoinduction by rhBMP-2: a histochemical and ultrastructural study. Information

Substance Name: Acid Phosphatase

Registry Number: EC 3.1.3.2

Grant and Affiliation Information for Characterization of the development of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells during osteoinduction by rhBMP-2: a histochemical and ultrastructural study.

AFFILIATION: Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Mie University, Tsu, Mie, Japan. toshi@clin.medic.mie-u.ac.jp

Country: Denmark

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Oral Dis

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization of the development of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells during osteoinduction by rhBMP-2: a histochemical and ultrastructural study Related Publications

• 8-Epi-iridodial glucoside from Boschniakia rossica.
• A direct colorimetric method for the determination of phospholipids with dithiocyanatoiron reagent.
• A simple method for measuring phosphatidylcholine as its hydrophobic complex with tetrathiocyanatocobaltate.
• A stabilizing factor of yeast mitochondrial F1F0-ATPase-inhibitor complex: common amino acid sequence with yeast ATPase inhibitor and E. coli epsilon and bovine delta subunits.
• Amino acid sequence of an intrinsic inhibitor of mitochondrial ATPase from yeast.
• An immuno-light- and electron-microscopic study of the expression of bone morphogenetic protein-2 during the process of ectopic bone formation in the rat.
• Binding of intrinsic ATPase inhibitor to mitochondrial ATPase--stoichiometry of binding of nucleotides, inhibitor, and enzyme.
• Binding properties of 9K protein to F1-ATPase: a counterpart ligand to the ATPase inhibitor.
• Binding properties of an intrinsic ATPase inhibitor and occurrence in yeast mitochondria of a protein factor which stabilizes and facilitates the binding of the inhibitor to F1F0-ATPase.
• Botulinum toxin treatment for blepharospasm associated with myasthenia gravis.
• Cell-free synthesis of mitochondrial ATPase inhibitor precursor and its transport into yeast mitochondria.
• Change in P-450 content accompanying aerobic formation of mitochondria in yeast.
• Characterization of the development of ectopic chondroid/bone matrix and chondrogenic/osteogenic cells during osteoinduction by rhBMP-2: a histochemical and ultrastructural study.
• Decrease in mitochondrial levels of adenine nucleotides and concomitant mitochondrial dysfunction in ischemic rat liver.
• Development of mitochondrial membranes in anaerobically grown yeast cells.
• Energy-independent protection of the oxidative phosphorylation capacity of mitochondria against anoxic damage by ATP and its non-metabolizable analogs.
• Existence of stoichiometric amounts of an intrinsic ATPase inhibitor and two stabilizing factors with mitochondrial ATP synthase in yeast.
• Hepatic accumulation of pyrophosphate during acetate metabolism.
• Interaction of mitochondrial aspartate aminotransferase with negatively charged lecithin liposomes.
• Involvement of Ca2+ release and activation of phospholipase A2 in mitochondrial dysfunction during anoxia.
• Kinetics of hydroperoxide degradation by NADP-glutathione system in mitochondria.
• Localization of cytochrome c peroxidase in anaerobically grown yeast cells.
• Non-mitochondrial electron transfer system in anaerobically grown yeast cells.
• Partial amino terminal sequence of the precursor of mitochondrial ATPase inhibitor protein synthesized with mRNA partially purified by gel permeation chromatography.
• Properties of binding sites for adenine nucleotides on ATPase from yeast mitochondria.
• Purification and properties of factors in yeast mitochondria stabilizing the F1F0-ATPase-inhibitor complex.
• Simultaneous bindings of ATPase inhibitor and 9K protein to F1F0-ATPase in the presence of 15K protein in yeast mitochondria.
• [Hypertension in dialysis patients]
• [Hypotension in dialysis patients]
• pH-Induced conformational change of ATPase inhibitor from yeast mitochondria. A proton magnetic resonance study.