Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues.

Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues. Research Abstract Details 

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Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues. Abstract Text:

B Spaulding,D Pan,A Ghadersohi,G Nielsen,S Jensen,F Gellert,X Ling,M Zhang,A Black,F Li,

AIMS: To elucidate the expression and regulation of survivin in normal tissues. METHODS AND RESULTS: A novel monoclonal antibody (12C4) to survivin was generated. Application of this antibody to determine survivin expression in human normal adult tissues revealed that most adult tissues do not express survivin and, where it is present, survivin is largely restricted to a small subset of epithelial cells and cells with proliferative potential such as thymus. Survivin expression among positive tissues showed individual variations, ranging from zero to < 5% positive cells in epithelial cell populations. Testis is the only human adult tissue highly expressing survivin, with 60-70% positivity in the nuclei of spermatogonia. Consistent with deregulated expression of survivin associated with oncogenesis, we found that certain ligands and transcription factors differentially modulate survivin promoter activity in cancer cells versus normal/untransformed cells. CONCLUSION: Deregulation of survivin transcription controls in individual epithelial cells may contribute to oncogenesis in various human adult tissues.

Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues. Publishing Authors By Initials

B Spaulding,D Pan,A Ghadersohi,G Nielsen,S Jensen,F Gellert,X Ling,M Zhang,A Black,F Li,

For similar investigative techniques: chemistry, analytical: microchip analytical procedures: microarray analysis: tissue array analysis research abstracts see: investigative techniques: chemistry, analytical: microchip analytical procedures: microarray analysis: tissue array analysis research

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Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Histopathology

VOLUME: 49

Page Numbers: 622-33

Journal Abbreviation: Histopathology

ISSN: 0309-0167

DAY: 3

MONTH: Dec

YEAR: 2006

Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7704136

Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues. Keywords Mesh Terms:

KEYWORDS: Tissue Array Analysis

MESH TERMS: metabolism

Chemical & Substance for Abstract: Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues. Information

Substance Name: Neoplasm Proteins

Registry Number: 0

Grant and Affiliation Information for Characterization of the 12C4 survivin monoclonal antibody and insight into the expression of survivin in human adult tissues.

AFFILIATION: Dako North America, Carpinteria, CA, USA.

Country: England

AGENCY: United States NCI

GRANT: CA16056

ACRONYM: CA

MEDLINETA: Histopathology

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