Characterization of terminal deoxynucleotidyl transferase and polymerase micro in zebrafish.

Characterization of terminal deoxynucleotidyl transferase and polymerase micro in zebrafish. Research Abstract Details 

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Characterization of terminal deoxynucleotidyl transferase and polymerase micro in zebrafish. Abstract Text:

Susann Beetz,Dagmar Diekhoff,Lisa A Steiner,Susann Beetz,Dagmar Diekhoff,Lisa A Steiner,

Terminal deoxynucleotidyl transferase (TdT) contributes to the junctional diversity of immunoglobulin and T-cell receptors by incorporating nucleotides in a template-independent manner. A closely related enzyme, polymerase micro (polmicro), a template-directed polymerase, plays a role in general end-joining double-strand break repair. We cloned zebrafish TdT and polmicro and found them to be 43% identical in amino acid sequence. Comparisons with sequences of other species revealed conserved residues typical for TdT in the zebrafish sequence that support the template independence of this enzyme. Some but not all of these features were identified in zebrafish polmicro. In adult fish, TdT expression was most prominent in thymus, pro- and mesonephros, the primary lymphoid organs in teleost fish and in spleen, intestine, and the tissue around the intestine. Polmicro expression was detected not only in pro- and mesonephros, the major sites for B-lymphocyte development, but also in ovary and testis and in all tissue preparations to a low extent. TdT expression starts at 4 dpf and increases thereafter. Polmicro is expressed at all times to a similar extent. In situ studies showed a strong expression of TdT and polmicro in the thymic cortex of 8-week-old fish. The characterization of zebrafish TdT and polmicro provide new insights in fish lymphopoiesis and addresses the importance and evolution of TdT and polmicro themselves.

Characterization of terminal deoxynucleotidyl transferase and polymerase micro in zebrafish. Publishing Authors By Initials

S Beetz,D Diekhoff,LA Steiner,S Beetz,D Diekhoff,LA Steiner,

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Characterization of terminal deoxynucleotidyl transferase and polymerase micro in zebrafish. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Immunogenetics

VOLUME: 59

Page Numbers: 735-44

Journal Abbreviation: Immunogenetics

ISSN: 0093-7711

DAY: 14

MONTH: 08

YEAR: 2007

Characterization of terminal deoxynucleotidyl transferase and polymerase micro in zebrafish. Information

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LANGUAGE: eng

NlmUniqueID: 420404

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Grant and Affiliation Information for Characterization of terminal deoxynucleotidyl transferase and polymerase micro in zebrafish.

AFFILIATION: Biology Department, Massachusetts Institute of Technology, Cambridge, MA, USA. beetz@immunologie.uni-kiel.de

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI08054

ACRONYM: AI

MEDLINETA: Immunogenetics

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