Characterization of telomerase-immortalized, non-neoplastic, human Barrett's cell line (BAR-T).

Characterization of telomerase-immortalized, non-neoplastic, human Barrett's cell line (BAR-T). Research Abstract Details 

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Characterization of telomerase-immortalized, non-neoplastic, human Barrett's cell line (BAR-T). Abstract Text:

K R Jaiswal,C P Morales,L A Feagins,K G Gandia,X Zhang,H-Y Zhang,K Hormi-Carver,Y Shen,F Elder,R D Ramirez,G A Sarosi,S J Spechler,R F Souza,

Barrett's esophagus, a metaplasia predisposed to malignant transformation, has been studied in vitro using esophageal adenocarcinoma cell lines. However, findings in such transformed cells may not be applicable to the non-neoplastic cells of benign Barrett's esophagus. Therefore, we have developed and characterized a Barrett's cell line derived from a patient without malignancy or dysplasia. Human Barrett's epithelial cells were immortalized with the insertion of hTERT (human telomerase reverse transcriptase) using a Cre-lox recombination system. We then examined properties of this continuous cell line, such as in vitro tumorigenicity, growth patterns, histological differentiation characteristics, karyotype, and checkpoint arrest mechanisms (e.g., p16, p21, and p53). Non-neoplastic Barrett's epithelial cells infected with hTERT (BAR-T cells) have been sustained in culture beyond 200 population doublings. BAR-T cells maintain a diploid chromosome number and exhibit non-neoplastic properties, such as contact inhibition and anchorage-dependent growth. BAR-T cells express differentiation Barrett's epithelial markers, such as villin and cytokeratins 4, 8 and 18, and stain positive for Alcian blue, indicating the presence of mucin-producing cells. Expression of checkpoint arrest proteins p21 and p53 are intact, while p16 expression is lost. Thus, we have created a human Barrett's cell line that is not malignantly transformed, and yet can be maintained indefinitely in culture. BAR-T cells are diploid, have histological differentiation markers characteristic of benign Barrett's epithelium, and also maintain appropriate expression of p21 and p53. This cell line should be a useful model for the study of the early events in carcinogenesis in Barrett's esophagus.

Characterization of telomerase-immortalized, non-neoplastic, human Barrett's cell line (BAR-T). Publishing Authors By Initials

KR Jaiswal,CP Morales,LA Feagins,KG Gandia,X Zhang,HY Zhang,K Hormi-Carver,Y Shen,F Elder,RD Ramirez,GA Sarosi,SJ Spechler,RF Souza,

For similar investigative techniques: genetic techniques: gene transfer techniques: transduction, genetic research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transduction, genetic research

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Characterization of telomerase-immortalized, non-neoplastic, human Barrett's cell line (BAR-T). Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Diseases of the esophagus : official journal of th

VOLUME: 20

Page Numbers: 256-64

Journal Abbreviation: Dis. Esophagus

ISSN: 1120-8694

DAY: 3

MONTH: 12

YEAR: 2007

Characterization of telomerase-immortalized, non-neoplastic, human Barrett's cell line (BAR-T). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8809160

Characterization of telomerase-immortalized, non-neoplastic, human Barrett's cell line (BAR-T). Keywords Mesh Terms:

KEYWORDS: Transduction, Genetic

MESH TERMS: physiology

Chemical & Substance for Abstract: Characterization of telomerase-immortalized, non-neoplastic, human Barrett's cell line (BAR-T). Information

Substance Name: Telomerase

Registry Number: EC 2.7.7.49

Grant and Affiliation Information for Characterization of telomerase-immortalized, non-neoplastic, human Barrett's cell line (BAR-T).

AFFILIATION: Department of Surgery, University of Texas Southwestern Medical Center at Dallas and the VA North Texas Healthcare System, Dallas, TX 75216, USA.

Country: Australia

AGENCY: United States NIDDK

GRANT: DK63621

ACRONYM: DK

MEDLINETA: Dis Esophagus

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