Characterization of site-specific recombination by the integrase MJ1 from enterococcal bacteriophage phiFC1.

Characterization of site-specific recombination by the integrase MJ1 from enterococcal bacteriophage phiFC1. Research Abstract Details 

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Characterization of site-specific recombination by the integrase MJ1 from enterococcal bacteriophage phiFC1. Abstract Text:

Mi-Ok Park,Ki-Hong Lim,Tae-Hyung Kim,Hyo-Ihl Chang,Mi-Ok Park,Ki-Hong Lim,Tae-Hyung Kim,Hyo-Ihl Chang,

Bacteriophage phiFC1 integrase (MJ1) was previously shown to perform a site-specific recombination between a phage attachment site (attP) and a host attachment site (attB) in its host, Enterococcus faecalis, and also in a non-host bacterium, Escherichia coli. Here, we investigated biochemical features of MJ1 integrase. First, MJ1 integrase could perform in vitro recombination between attP and attB in the absence of additional factors. Second, MJ1 integrase interacted with att sites. Electrophoretic mobility shift assays and DNase I footprinting revealed that MJ1 integrase could efficiently bind to all the att sites and that MJ1 integrase recognized relatively short sequences (approximately 50 bp) containing an overlapping region within attB and attP. These results demonstrate that MJ1 integrase indeed catalyzes an integrative recombination between attP and attB, the mechanism of which might be simple and unidirectional, as found in serine integrases.

Characterization of site-specific recombination by the integrase MJ1 from enterococcal bacteriophage phiFC1. Publishing Authors By Initials

MO Park,KH Lim,TH Kim,HI Chang,MO Park,KH Lim,TH Kim,HI Chang,

For similar genetic processes: recombination, genetic research abstracts see: genetic processes: recombination, genetic research

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Characterization of site-specific recombination by the integrase MJ1 from enterococcal bacteriophage phiFC1. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of microbiology and biotechnology

VOLUME: 17

Page Numbers: 342-7

Journal Abbreviation: J. Microbiol. Biotechnol.

ISSN: 1017-7825

DAY: 3

MONTH: Feb

YEAR: 2007

Characterization of site-specific recombination by the integrase MJ1 from enterococcal bacteriophage phiFC1. Information

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LANGUAGE: eng

NlmUniqueID: 9431852

Characterization of site-specific recombination by the integrase MJ1 from enterococcal bacteriophage phiFC1. Keywords Mesh Terms:

KEYWORDS: Recombination, Genetic

MESH TERMS: physiology

Chemical & Substance for Abstract: Characterization of site-specific recombination by the integrase MJ1 from enterococcal bacteriophage phiFC1. Information

Substance Name: Integrases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Characterization of site-specific recombination by the integrase MJ1 from enterococcal bacteriophage phiFC1.

AFFILIATION: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.

Country: Korea (South)

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MEDLINETA: J Microbiol Biotechnol

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