Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities.

Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities. Abstract Text:

Kazunori Goto,Takashi Kobori,Yoshitaka Kosaka,Tomohide Natsuaki,Chikara Masuta,

Double-stranded (ds) RNAs and imperfect hairpin RNAs of endogenous genes trigger post-transcriptional gene silencing (PTGS) and are cleaved by a Dicer-like nuclease into small interfering RNAs (siRNAs) and microRNs (miRNAs), respectively. Such small RNAs (siRNAs and miRNAs) then guide an RNA-induced silencing complex (RISC) for sequence-specific RNA degradation. While PTGS serves as an antiviral defense in plants, many plant viruses encode suppressors as a counter defense. Here we demonstrate that the PTGS suppressor (2b) of a severe strain (CM95R) of cucumber mosaic virus (CMV) can bind to in vitro synthesized siRNAs and even to long dsRNAs to a lesser extent. However, the 2b suppressor weakly bound to a miRNA (miR171) duplex in contrast to another small RNA-binding suppressor, p19 of tombusvirus that can effectively bind miRNAs. Because the 2b suppressor of an attenuated strain of CMV (CM95), which differs in a single amino acid from the 2b of CM95R, could barely bind siRNAs, we hypothesized that the weak suppressor activity of the attenuated strain resulted from a loss of the siRNA-binding property of 2b via a single amino acid change. Here we consider that 2b interferes with the PTGS pathway by directly binding siRNAs (or long dsRNA).

Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities. Publishing Authors By Initials

K Goto,T Kobori,Y Kosaka,T Natsuaki,C Masuta,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

PUBMED ID PMID:

MEDLINE DATE:

Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Plant & cell physiology

VOLUME: 48

Page Numbers: 1050-60

Journal Abbreviation: Plant Cell Physiol.

ISSN: 0032-0781

DAY: 13

MONTH: 06

YEAR: 2007

Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9430925

Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities. Information

Substance Name: Viral Proteins

Registry Number: 0

Grant and Affiliation Information for Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities.

AFFILIATION: Cell Biology and Manipulation Laboratory, Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589 Japan.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Plant Cell Physiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities Related Publications

• Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities.
• Fine genetic mapping of the TuNI locus causing systemic veinal necrosis by turnip mosaic virus infection in Arabidopsis thaliana.