Characterization of rickettsial infection in Amblyomma americanum (Acari: Ixodidae) by quantitative real-time polymerase chain reaction.

Characterization of rickettsial infection in Amblyomma americanum (Acari: Ixodidae) by quantitative real-time polymerase chain reaction. Research Abstract Details 

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Characterization of rickettsial infection in Amblyomma americanum (Acari: Ixodidae) by quantitative real-time polymerase chain reaction. Abstract Text:

Several species of the spotted fever group Rickettsia (SFGR), with considerable variation in vertebrate host pathogenicity, are present in ticks in the United States. In this study, quantitative real-time PCR (qPCR) was used to characterize the growth and the distribution of Rickettsia amblyommii in selected tissues (salivary glands, gut, and ovaries) of naturally infected Amblyomma americanum (L.) (Acari: Ixodidae), during bloodmeal acquisition and throughout vertical transmission to eggs and postembryonic life cycle stages (larvae and nymphs). R. amblyommii was identified in the samples at ratios of < or = 1 rickettsiae per tick cell. Significant variability in the ratio of rickettsial to tick gene copy numbers between the tissues was identified; however, no single tissue was consistently observed to have the greatest rickettsial burden throughout the feeding event. Furthermore, the ratio of rickettsial to tick gene copy numbers did not significantly differ between eggs, immature ticks, and feeding events. This is the first study to use qPCR to enumerate rickettsial growth and distribution in the tick host during bloodmeal acquisition. Deciphering SFGR tissue distribution and transmission mechanisms is necessary for the development of novel approaches to control tick-borne rickettsial diseases.

Characterization of rickettsial infection in Amblyomma americanum (Acari: Ixodidae) by quantitative real-time polymerase chain reaction. Publishing Authors By Initials

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Characterization of rickettsial infection in Amblyomma americanum (Acari: Ixodidae) by quantitative real-time polymerase chain reaction. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of medical entomology

VOLUME: 45

Page Numbers: 267-75

Journal Abbreviation: J. Med. Entomol.

ISSN: 0022-2585

DAY: 11

MONTH: Mar

YEAR: 2008

Characterization of rickettsial infection in Amblyomma americanum (Acari: Ixodidae) by quantitative real-time polymerase chain reaction. Information

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LANGUAGE: eng

NlmUniqueID: 375400

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Grant and Affiliation Information for Characterization of rickettsial infection in Amblyomma americanum (Acari: Ixodidae) by quantitative real-time polymerase chain reaction.

AFFILIATION: Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman Drive, SVM-3213, Baton Rouge, LA 70803 , USA.

Country: United States

AGENCY: United States NCRR

GRANT: P20 RR0201595

ACRONYM: RR

MEDLINETA: J Med Entomol

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