[Characterization of resistant cell line of Trichoplusia ni to Bacillus thuringiensis toxin Cry1Ac]

[Characterization of resistant cell line of Trichoplusia ni to Bacillus thuringiensis toxin Cry1Ac] Research Abstract Details 

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[Characterization of resistant cell line of Trichoplusia ni to Bacillus thuringiensis toxin Cry1Ac] Abstract Text:

Kai Yu Liu,Hong Yang,Cai Fu Jiang,Jian Xin Peng,Hua Zhu Hong,

The cell line (BTI-TN-5B1-4) of Trichoplusia ni had been selected by Bacilllus thuringiensis (Bt) toxin Cry1Ac for 86 generations continuously. A high level of resistance was achieved and the partial characterizations of the resistance-selected BTI-TN-5B1-4 cells were studied in order to explore the mechanism of the resistance to Bt toxin. The cells selected by Cry1Ac had low level of cross-resistance (19.7 fold) to Cry1C, their ability of tolerance in the hypotonic solution increased evidently (2.5 fold), and there were differential membrane proteins between resistance-selected and unselected susceptible cells on two dimensional electrophoresis maps. We supposed the changes of membrane proteins in resistant cells would be linked to the cross-resistance to Cry1C and the increase of tolerance in hypotonic solution.

[Characterization of resistant cell line of Trichoplusia ni to Bacillus thuringiensis toxin Cry1Ac] Publishing Authors By Initials

KY Liu,H Yang,CF Jiang,JX Peng,HZ Hong,

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[Characterization of resistant cell line of Trichoplusia ni to Bacillus thuringiensis toxin Cry1Ac] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Shi yan sheng wu xue bao

VOLUME: 37

Page Numbers: 205-11

Journal Abbreviation: Shi Yan Sheng Wu Xue Bao

ISSN: 0001-5334

DAY: 24

MONTH: Jun

YEAR: 2004

[Characterization of resistant cell line of Trichoplusia ni to Bacillus thuringiensis toxin Cry1Ac] Information

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LANGUAGE: chi

NlmUniqueID: 413570

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Grant and Affiliation Information for [Characterization of resistant cell line of Trichoplusia ni to Bacillus thuringiensis toxin Cry1Ac]

AFFILIATION: Institute of Entomology, Central China Normal University, Wuhan 430079, China.

Country: China

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MEDLINETA: Shi Yan Sheng Wu Xue Bao

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