Characterization of regulatory light chain-a myosin kinase from smooth muscle of scallop, Patinopecten yessoensis.

Characterization of regulatory light chain-a myosin kinase from smooth muscle of scallop, Patinopecten yessoensis. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of regulatory light chain-a myosin kinase from smooth muscle of scallop, Patinopecten yessoensis. Abstract Text:

H Sohma,F Morita,

The protein kinase that phosphorylates the regulatory light chain-a (RLC-a) of scallop smooth muscle myosin was isolated from scallop smooth muscle (Sohma, H. & Morita, F. (1986) J. Biochem. 100, 1155-1163). The enzymatic properties of this kinase (aMK) were investigated using RLC-a as the substrate. The Km value for ATP was 6.5 microM in the presence of 27 microM RLC-a at pH 7.0, and that for RLC-a was 133 microM in the presence of 1 mM ATP. The Vm value at saturation of both RLC-a and ATP was 0.25 s-1 at pH 7.0. The pH activity curve for aMK was bell-shaped with a maximum at around pH 7.8. The aMK activity was inhibited strongly by an increase in the KCl concentration. aMK required Mg2+, but was inhibited by high concentrations of Mg2+. The optimum activity was seen at 3 mM MgCl2. The mode of inhibition of the aMK activity by Ca2+ was studied. Assuming that the binding of Ca2+ to aMK induces the inhibition, the dissociation constant of Ca2+ was estimated to be 64 microM. aMK also phosphorylated LC20 of chicken gizzard myosin at a similar rate to that for RLC-a and the DTNB light chain of rabbit skeletal muscle myosin at a more lower rate. The helix and beta-sheet contents of aMK were estimated to be 19 and 30%, respectively, from the CD spectrum.

Characterization of regulatory light chain-a myosin kinase from smooth muscle of scallop, Patinopecten yessoensis. Publishing Authors By Initials

H Sohma,F Morita,

For similar investigative techniques: chemistry, analytical: photometry: spectrophotometry: spectrophotometry, ultraviolet research abstracts see: investigative techniques: chemistry, analytical: photometry: spectrophotometry: spectrophotometry, ultraviolet research

PUBMED ID PMID:

MEDLINE DATE:

Characterization of regulatory light chain-a myosin kinase from smooth muscle of scallop, Patinopecten yessoensis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 101

Page Numbers: 497-502

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1987

Characterization of regulatory light chain-a myosin kinase from smooth muscle of scallop, Patinopecten yessoensis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Characterization of regulatory light chain-a myosin kinase from smooth muscle of scallop, Patinopecten yessoensis. Keywords Mesh Terms:

KEYWORDS: Spectrophotometry, Ultraviolet

MESH TERMS: metabolism

Chemical & Substance for Abstract: Characterization of regulatory light chain-a myosin kinase from smooth muscle of scallop, Patinopecten yessoensis. Information

Substance Name: Myosin-Light-Chain Kinase

Registry Number: EC 2.7.1.117

Grant and Affiliation Information for Characterization of regulatory light chain-a myosin kinase from smooth muscle of scallop, Patinopecten yessoensis.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization of regulatory light chain-a myosin kinase from smooth muscle of scallop, Patinopecten yessoensis Related Publications

• Association between experience of stressful life events and muscle-related temporomandibular disorders in patients seeking free treatment in a dental hospital.
• Cerebral hemodynamics during microgravity.
• Characterization of Lactococcus garvieae isolated from radish and broccoli sprouts that exhibited a KG+ phenotype, lack of virulence and absence of a capsule.
• Cryptadines A and B, novel C(27)N(3)-type pentacyclic alkaloids from Lycopodium cryptomerinum.
• Degradation of arylarsenic compounds by microorganisms.
• Development of a fully automatic scheme for detection of masses in whole breast ultrasound images.
• Efficacy, safety, and cost effectiveness of intravenous midazolam and flunitrazepam for primary insomnia in terminally ill patients with cancer: a retrospective multicenter audit study.
• Features and feedback: enhancing metamnemonic knowledge at retrieval reduces source-monitoring errors.
• Fusion of single-photon emission computed tomography and computed tomography images of sentinel lymph nodes in extramammary Paget's disease of the scrotum.
• Gait analysis in an osteoarthritis patient with restoration of the hip joint space following contralateral total hip arthroplasty: a case report.
• Glucose intolerance during hormonal therapy for prostate cancer.
• High salt culture conditions inhibit serum- and NGF- but not PMA-induced Egr-1 gene transcription in rat C6 glioma cells.
• Identification and characterization of RNA-dependent RNA polymerase activity in recombinant Japanese encephalitis virus NS5 protein.
• Molecular dynamics analysis of interfacial structures and sum frequency generation spectra of aqueous hydrogen halide solutions.
• Novel functions of herbal medicines in dendritic cells: role of amomi semen in tumor immunity.
• Plastic alteration of vestibulo-cardiovascular reflex induced by 2 weeks of 3-G load in conscious rats.
• Polygoacetophenoside, A New Acetophenone Glucoside from Polygonum multiflorum1.
• Pordamacrines A and B, alkaloids from Daphniphyllum macropodum.
• Preferential recognition of a microbial metabolite by human Vgamma2Vdelta2 T cells.
• Protein kinase CK2 is a key activator of histone deacetylase in hypoxia-associated tumors.
• Range of motion measurement of an artificial hip joint using CT images.
• Regulation of bone morphogenetic protein-2 expression by endogenous prostaglandin E2 in human mesenchymal stem cells.
• Snake venom components affecting blood coagulation and the vascular system: structural similarities and marked diversity.
• Taxodistines A and B, abietane-type diterpenes from Taxodium distichum.
• Tobacco smoke causes premature skin aging.
• Transforming growth factor-beta stimulates IL-1beta-induced monocyte chemoattractant protein-1 expression in human synovial cells via the ERK/AP-1 pathway.
• [Pericardial cyst]
• [Protective role of HO-1 in oxidative tissue injuries]
• [Recent changes in the incidence of life-threatening events in the operating room: JSA surveys between 2001 and 2005]
• [Resection of cancer of the cardia enabled by combined treatment with S-1 and paclitaxel after esophageal stenting for impaired patency complicating stage IV gastric cancer - a case report]