Characterization of RagA and RagB in Porphyromonas gingivalis: study using gene-deletion mutants.

Characterization of RagA and RagB in Porphyromonas gingivalis: study using gene-deletion mutants. Research Abstract Details 

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Characterization of RagA and RagB in Porphyromonas gingivalis: study using gene-deletion mutants. Abstract Text:

Keiji Nagano,Yukitaka Murakami,Kiyoshi Nishikawa,Junpei Sakakibara,Kazuo Shimozato,Fuminobu Yoshimura,Keiji Nagano,Yukitaka Murakami,Kiyoshi Nishikawa,Junpei Sakakibara,Kazuo Shimozato,Fuminobu Yoshimura,Keiji Nagano,Yukitaka Murakami,Kiyoshi Nishikawa,Junpei Sakakibara,Kazuo Shimozato,Fuminobu Yoshimura,

The major outer-membrane proteins RagA and RagB of Porphyromonas gingivalis are considered to form a receptor complex functionally linked to TonB. In this study, P. gingivalis mutants with ragA, ragB or both deleted were constructed from strain W83 as the parent to examine the physiological and pathological functions of RagA and RagB. The double-deletion mutant completely lacked both RagA and RagB, whereas the DeltaragA mutant reduced RagB expression considerably and the DeltaragB mutant produced degraded RagA. Growth of the three mutants in a nutrient-rich medium and synthetic media containing digested protein as a unique nutrient source was similar to that of the parental strain; however, both the DeltaragA and DeltaragAB mutants exhibited very slow growth in a synthetic medium containing undigested, native protein, and the two mutants tended to lose their viability during experiments, although gingipain (protease) activities were unchanged in the mutants. A mouse model showed that the DeltaragB mutant had reduced virulence. Cell-surface labelling with biotin and dextran revealed that both RagA and RagB localized on the outermost cell surface. A cross-linking experiment using wild-type P. gingivalis showed that RagA and RagB were closely associated with each other. Furthermore, co-immunoprecipitation confirmed that RagA and RagB formed a protein-protein complex. These results suggest that physically associated RagA and RagB may stabilize themselves on the cell surface and function as active transporters of large degradation products of protein and in part as a virulence factor.

Characterization of RagA and RagB in Porphyromonas gingivalis: study using gene-deletion mutants. Publishing Authors By Initials

K Nagano,Y Murakami,K Nishikawa,J Sakakibara,K Shimozato,F Yoshimura,K Nagano,Y Murakami,K Nishikawa,J Sakakibara,K Shimozato,F Yoshimura,K Nagano,Y Murakami,K Nishikawa,J Sakakibara,K Shimozato,F Yoshimura,

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Characterization of RagA and RagB in Porphyromonas gingivalis: study using gene-deletion mutants. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of medical microbiology

VOLUME: 56

Page Numbers: 1536-48

Journal Abbreviation: J. Med. Microbiol.

ISSN: 0022-2615

DAY: 29

MONTH: Nov

YEAR: 2007

Characterization of RagA and RagB in Porphyromonas gingivalis: study using gene-deletion mutants. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Characterization of RagA and RagB in Porphyromonas gingivalis: study using gene-deletion mutants.

AFFILIATION: 1Department of Microbiology, School of Dentistry, Aichi-Gakuin University, 1-100 Kusumoto-cho, Chikusa-ku, Nagoya, Aichi 464-8650, Japan.

Country: England

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MEDLINETA: J Med Microbiol

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