Characterization of mucins from cultured normal human tracheobronchial epithelial cells.

Characterization of mucins from cultured normal human tracheobronchial epithelial cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of mucins from cultured normal human tracheobronchial epithelial cells. Abstract Text:

D J Thornton,T Gray,P Nettesheim,M Howard,J S Koo,J K Sheehan,

Early-passage normal human tracheobronchial epithelial (NHTBE) cells grown in air-liquid interface cultures in medium containing retinoids differentiate into a mucociliary epithelium over a 2- to 3-wk period and express increasing mRNA levels of the airway mucin genes MUC5AC and MUC5B as the cultures age; the levels of MUC2 mRNA were very low throughout the study. Using specific antibodies to MUC5AC and MUC5B mucins, we noted a gradual increase in these two mucins in the intracellular and apically secreted pools as a function of time. A low level of MUC2 mucin was detected, which did not change with time. The intracellular and apically secreted mucins isolated from day 14 and day 21 cultures by density gradient centrifugation were similar in density to those previously isolated from human respiratory mucus secretions. The sedimentation rate of the apically secreted mucins indicated that they were highly oligomerized, polydisperse macromolecules similar to those previously documented from in vivo secretions. In contrast, the cell-associated mucins from the cultured NHTBE cells were much smaller, possibly only monomers and dimers. Anion-exchange chromatography detected no differences in charge density between the reduced and carboxymethylated cell-associated and secreted forms of the MUC5AC and MUC5B mucins. The MUC5AC mucin was of similar charge density to its in vivo counterpart; however, MUC5B was more homogeneous than that found in vivo. Finally, evidence is presented for an intracellular NH(2)-terminal cleavage of the MUC5B mucins. These studies indicate that the mucins produced by cultured NHTBE cells are similar to those found in human airways, suggesting that this cell culture model is suited for studies of respiratory mucin biosynthesis, processing, and assembly.

Characterization of mucins from cultured normal human tracheobronchial epithelial cells. Publishing Authors By Initials

DJ Thornton,T Gray,P Nettesheim,M Howard,JS Koo,JK Sheehan,

For similar respiratory system: trachea research abstracts see: respiratory system: trachea research

PUBMED ID PMID:

MEDLINE DATE:

Characterization of mucins from cultured normal human tracheobronchial epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Lung cellular and

VOLUME: 278

Page Numbers: L1118-28

Journal Abbreviation: Am. J. Physiol. Lung Cell Mol.

ISSN: 1040-0605

DAY: 29

MONTH: Jun

YEAR: 2000

Characterization of mucins from cultured normal human tracheobronchial epithelial cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901229

Characterization of mucins from cultured normal human tracheobronchial epithelial cells. Keywords Mesh Terms:

KEYWORDS: Trachea

MESH TERMS: secretion

Chemical & Substance for Abstract: Characterization of mucins from cultured normal human tracheobronchial epithelial cells. Information

Substance Name: mucin 5AC

Registry Number: 0

Grant and Affiliation Information for Characterization of mucins from cultured normal human tracheobronchial epithelial cells.

AFFILIATION: The Wellcome Trust Centre for Cell-Matrix Research, Division of Biochemistry, School of Biological Sciences, University of Manchester, United Kingdom. Dave.Thorton@man.ac.uk

Country: UNITED STATES

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Am J Physiol Lung Cell Mol Phy

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization of mucins from cultured normal human tracheobronchial epithelial cells Related Publications

• A comparison of diabetic foot ulcer outcomes using negative pressure wound therapy versus historical standard of care.
• A new vision for an aging population. The church can play a major role.
• A study of the intracellular and secreted forms of the MUC2 mucin from the PC/AA intestinal cell line.
• Alcohol-based instant hand sanitizer use in military settings: a prospective cohort study of Army basic trainees.
• Arthroplasty of the knee.
• Characterization of mucins from cultured normal human tracheobronchial epithelial cells.
• Concentrated solutions of salivary MUC5B mucin do not replicate the gel-forming properties of saliva.
• Cumulative asbestos exposure for US automobile mechanics involved in brake repair (circa 1950s-2000).
• Current concepts in management of meningiomas and schwannomas.
• D-phenylglycinol-derived non-covalent factor Xa inhibitors: effect of non-peptidic S4 linkage elements on affinity and anticoagulant activity.
• Evaluation of a Repeat Sprint Test for Australian Football: Relationship to Speed and Endurance: 1443: Board #206 May 30 9:30 AM - 11:00 AM.
• Foam cell plaques in intima of myometrial arteries; a presumptive sign of previous irradiation.
• Identification of a nonmucin glycoprotein (gp-340) from a purified respiratory mucin preparation: evidence for an association involving the MUC5B mucin.
• Identification of two glycoforms of the MUC5B mucin in human respiratory mucus. Evidence for a cysteine-rich sequence repeated within the molecule.
• Optic atrophy with altitudinal hemianopia in neurofibromatosis.
• Paroxysmal haemoglobinuria with a report on three cases.
• Pediatric advocacy.
• Preliminary study pointing out a significant alteration in the biochemical composition of MUC2 in colorectal mucinous carcinoma.
• Proteomic identification of tyrosine nitration targets in kidney of spontaneously hypertensive rats.
• Redox proteomics in the mussel, Mytilus edulis.
• Retained placenta and postpartum haemorrhage.
• Salivary mucin MG1 is comprised almost entirely of different glycosylated forms of the MUC5B gene product.
• Simulation tests to assess occupational exposure to airborne asbestos from asphalt-based roofing products.
• Spatial analysis of colorectal cancer incidence and proportion of late-stage in Massachusetts residents: 1995-1998.
• Spleen cell phosphorylation of salt soluble nuclear protein from isoproterenol treated and sorted nuclei.
• The efficacy of vaginal clindamycin for the treatment of abnormal genital tract flora in pregnancy.
• The heparin-binding exosite is critical to allosteric activation of factor IXa in the intrinsic tenase complex: the role of arginine 165 and factor X.
• The potential of proteomics for providing new insights into environmental impacts on human health.
• The silence of suffering: breaking the sound barriers.
• Three-dimensional object representation and invariant recognition using continuous distance transform neural networks.