Characterization of human erythrocyte cytoskeletal ATPase.

Characterization of human erythrocyte cytoskeletal ATPase. Research Abstract Details 

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Characterization of human erythrocyte cytoskeletal ATPase. Abstract Text:

S Sato,Y Jinbu,M Nakao,

Human erythrocyte cytoskeletal ATPase was extracted with 0.2 mM ATP (pH 8.0) from Triton X-100 treated ghosts. The ATPase fraction contained mainly spectrin, actin, and band 4.1. When the ATPase fraction was applied to a Sepharose 4B column, 90% of the ATPase activity was recovered in a spectrin, actin, and band 4.1 complex fraction and none was detected in the spectrin fraction. A specific activity of the complex ATPase was 60-120 nmol/(mg protein X h). No ATPase activity was detected in the presence of EDTA. The presence of magnesium in the incubation medium was essential for the ATPase activity. The activity was activated by KCl and was almost completely inhibited by 10(-5) M free calcium in the presence of 0.2 mM MgCl2. The Ki for Ca2+ was 7 X 10(-7) M. Phalloidin and DNase 1, which affect actin, inhibited this K,Mg-ATPase activity by 95%, but cytochalasin B did not inhibit it. N-Ethylmaleimide activated the ATPase 1.6-fold. The order of affinity for nucleotides was ATP greater than ITP greater than CTP, ADP, AMP-PNP, GTP. A specific ATPase activity of purified actin was 50 nmol/(mg X h). These results suggest that the cytoskeletal ATPase is actin ATPase and the actin ATPase is activated by spectrin and band 4.1.

Characterization of human erythrocyte cytoskeletal ATPase. Publishing Authors By Initials

S Sato,Y Jinbu,M Nakao,

For similar chlorides: potassium chloride research abstracts see: chlorides: potassium chloride research

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Characterization of human erythrocyte cytoskeletal ATPase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 100

Page Numbers: 643-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1986

Characterization of human erythrocyte cytoskeletal ATPase. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Characterization of human erythrocyte cytoskeletal ATPase. Keywords Mesh Terms:

KEYWORDS: Potassium Chloride

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Characterization of human erythrocyte cytoskeletal ATPase. Information

Substance Name: Adenosine Triphosphatases

Registry Number: EC 3.6.1.-

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Country: JAPAN

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MEDLINETA: J Biochem

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