Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae).

Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae). Research Abstract Details 

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Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae). Abstract Text:

Fiorenzo Stirpe,Andrea Bolognesi,Massimo Bortolotti,Valentina Farini,Chiara Lubelli,Emanuele Pelosi,Letizia Polito,Barbara Dozza,Paola Strocchi,Angela Chambery,Augusto Parente,Luigi Barbieri,

From the caudices of the Passifloraceae Adenia lanceolata and A. stenodactyla, two lectins called lanceolin and stenodactylin, respectively, were purified by affinity chromatography on CL Sepharose 6B. The lectins are glycoproteins with M(r) 61,243 (lanceolin) and 63,131 (stenodactylin), consisting of an enzymatic A chain linked to a larger B chain with lectin properties, with N-terminal amino acid sequences similar to that of volkensin, the toxic lectin from A. volkensii. The lectins agglutinate red blood cells, inhibit protein synthesis both by a cell-free system and by whole cells, and depurinate ribosomes and DNA, but not tRNA or poly(A). They are highly toxic to cells, in which they induce apoptosis, and to mice, with LD(50)s 8.16mug/kg (lanceolin) and 2.76mug/kg (stenodactylin) at 48h. Thus, lanceolin and stenodactylin have all the properties of the toxic type 2 ribosome-inactivating proteins and are amongst the most potent toxins of plant origin.

Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae). Publishing Authors By Initials

F Stirpe,A Bolognesi,M Bortolotti,V Farini,C Lubelli,E Pelosi,L Polito,B Dozza,P Strocchi,A Chambery,A Parente,L Barbieri,

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Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Toxicon : official journal of the International So

VOLUME: 50

Page Numbers: 94-105

Journal Abbreviation: Toxicon

ISSN: 0041-0101

DAY: 7

MONTH: 03

YEAR: 2007

Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae). Information

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LANGUAGE: eng

NlmUniqueID: 1307333

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AFFILIATION: Dipartimento di Patologia sperimentale, Università di Bologna, I-40126 Bologna, Italy.

Country: England

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MEDLINETA: Toxicon

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