Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons.

Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons. Abstract Text:

Kazuki Nagasawa,Fumio Kawasaki,Akiko Tanaka,Katsuhito Nagai,Sadaki Fujimoto,Kazuki Nagasawa,Fumio Kawasaki,Akiko Tanaka,Katsuhito Nagai,Sadaki Fujimoto,

In this study, we examined the transport mechanisms for guanine and guanosine in rat neurons and astrocytes, and compared their characteristics. In the both types of cell, the uptake of [(3)H]guanine and [(3)H]guanosine was time-, temperature-, and concentration-dependent, and Na(+)-independent. Their uptake decreased on the addition of purine and pyrimidine nucleobases or nucleosides, and the inhibitory effect of the purine analogues was greater than that of the pyrimidine ones. In both cell types, equilibrative nucleoside transporter (ENT) 1 and ENT2 expression was confirmed at the mRNA level, and nitrobenzylmercaptopurine riboside, a representative inhibitor for ENT, decreased their uptake at concentrations of over 10 microM. Comparing uptake characteristics between the substrates, [(3)H]guanine uptake exhibited higher affinity and clearance than [(3)H]guanosine uptake in each type of cell. Although between neurons and astrocytes, there was no difference in the apparent uptake clearance for [(3)H]guanine and [(3)H]guanosine, which was calculated based upon the cellular protein content, the cellular uptake clearance was significantly greater in astrocytes than in neurons. These findings indicate that guanine and guanosine, of which the former is a preferable substrate, are taken up into both neurons and astrocytes via ENT2, and that the extracellular concentrations of guanine and guanosine are mainly regulated by astrocytes to maintain brain physiology.

Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons. Publishing Authors By Initials

K Nagasawa,F Kawasaki,A Tanaka,K Nagai,S Fujimoto,K Nagasawa,F Kawasaki,A Tanaka,K Nagai,S Fujimoto,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Glia

VOLUME: 55

Page Numbers: 1397-404

Journal Abbreviation: Glia

ISSN: 0894-1491

DAY: 1

MONTH: Nov

YEAR: 2007

Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8806785

Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons.

AFFILIATION: Department of Environmental Biochemistry, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto, Japan. nagasawa@mb.kyoto-phu.ac.jp

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Glia

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons Related Publications

• A 12 month in vivo study on the response of bone to a hydroxyapatite-polymethylmethacrylate cranioplasty composite.
• Asian ethnicity is associated with reduced pregnancy outcomes after assisted reproductive technology.
• Buffered Fourier domain mode locking: Unidirectional swept laser sources for optical coherence tomography imaging at 370,000 lines/s.
• Characterization of guanine and guanosine transport in primary cultured rat cortical astrocytes and neurons.
• Cinnamylindoline derivatives: synthesis and factor Xa (FXa) inhibitory activities.
• Comparison of Entamoeba histolytica DNA isolated from a cynomolgus monkey with human isolates.
• Developmental status of Aquatic Animal Experiment Facility, Aquatic Habitat (AQH), for International Space Station.
• Differentiation of gastric surface mucous cells (GSM06) induced by air-liquid interface is regulated partly through mitogen-activated protein kinase pathway.
• Diode-pumped, high-average-power femtosecond Cr(3+):LiCAF laser.
• Effective two-color SNP typing based on photoligation.
• Fourier domain mode locking at 1050 nm for ultra-high-speed optical coherence tomography of the human retina at 236,000 axial scans per second.
• Hepatocyte growth factor promotes remodeling of murine liver fibrosis, accelerating recruitment of bone marrow-derived cells into the liver.
• High-dose vitamins E and C supplementation prevents ribavirin-induced hemolytic anemia in patients with chronic hepatitis C.
• High-resolution line-scanning optical coherence microscopy.
• High-resolution three-dimensional optical coherence tomography imaging of kidney microanatomy ex vivo.
• Measuring dietary acculturation in Japanese Americans with the use of confirmatory factor analysis of food-frequency data.
• Molecular cloning and functional characterization of a prolactin-releasing peptide homolog from Xenopus laevis.
• New beamline optics for the effective use of a wiggler beam.
• PC-based rehabilitation tool for the elderly.
• Parallel processable light-driven DNA logic gate.
• Paraneoplastic dermatosis suspected of being Bazex syndrome in a woman with breast cancer.
• Percutaneous radiofrequency ablation and transcatheter arterial chemoembolization for hypervascular hepatocellular carcinoma: rate and risk factors for local recurrence.
• Phase-sensitive optical coherence tomography at up to 370,000 lines per second using buffered Fourier domain mode-locked lasers.
• Photochemical synthesis of R-shaped DNA via 5-cyanovinyldeoxyuridine.
• Photochemical transition of 5-methylcytosine to thymine by DNA photoligation.
• Polyester non-woven fabric finger cover as a TRUCT Braille reading assistance tool for Braille learners.
• Quantitative retention of membrane lipids in the freeze-fracture replica.
• Sequence specific interstrand photocrosslinking for effective SNP typing.
• The hydrogen bonding and amino-imino tautomerization of the alkoxy-aminopyridines and amino-methoxypyrimidines with acetic acid The effects of the methoxy group.
• Ultralow-threshold Kerr-lens mode-locked Ti:Al(2)O(3) laser.