Characterization of growth and osteogenic differentiation of rabbit bone marrow stromal cells.

Characterization of growth and osteogenic differentiation of rabbit bone marrow stromal cells. Research Abstract Details 

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Characterization of growth and osteogenic differentiation of rabbit bone marrow stromal cells. Abstract Text:

Jason Roostaeian,Brian Carlsen,David Simhaee,Reza Jarrahy,Weibiao Huang,Kenji Ishida,George H Rudkin,Dean T Yamaguchi,Timothy A Miller,

BACKGROUND: The rabbit is recognized as an excellent model to study the repair of bony defects with tissue engineered constructs. However, the use of rabbit bone marrow stromal cells (RBMSCs) has been limited despite the proven benefits of autologous BMSCs in the formation of bone. The purpose of this study was to characterize the growth and differentiation pattern of RBMSCs and their response to growth factors. MATERIAL AND METHODS: BMSCs were isolated from New Zealand White rabbits and cultured. Serial cell counts of parallel cultures were taken daily to determine cell growth. Response of RBMSCs to varying doses of recombinant human BMP-2 (rhBMP-2) and their time course was gauged by alkaline phosphatase (ALP) activity. The osteoblastic differentiation potential of RBMSCs in response to rhBMP-2 treatment was determined by evaluating the expression pattern of various genes involved with osteogensis using northern analysis. Von Kossa staining was performed to determine the effect of rhBMP-2 on the mineralization capabilities of RBMSCs. RESULTS: The growth rate of RBMSCs severely declined after first passage and this rate was further suppressed by TGF-beta1. The optimal dose response of rhBMP-2 was determined to be 50 ng/ml and its time course displayed increasing alkaline phosphatase activity over time. Two osteogenic markers, collagen I and osteopontin, were up regulated by rhBMP-2 treatment. Finally, the mineralization capability of RBMSCs was determined to be enhanced by rhBMP-2 treatment. CONCLUSION: Our work indicates that RBMSCs possess strong osteogenic potential and can be successfully applied toward bone tissue engineering in a rabbit model.

Characterization of growth and osteogenic differentiation of rabbit bone marrow stromal cells. Publishing Authors By Initials

J Roostaeian,B Carlsen,D Simhaee,R Jarrahy,W Huang,K Ishida,GH Rudkin,DT Yamaguchi,TA Miller,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta: transforming growth factor beta1 research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta: transforming growth factor beta1 research

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Characterization of growth and osteogenic differentiation of rabbit bone marrow stromal cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of surgical research

VOLUME: 133

Page Numbers: 76-83

Journal Abbreviation: J. Surg. Res.

ISSN: 0022-4804

DAY: 19

MONTH: 12

YEAR: 2005

Characterization of growth and osteogenic differentiation of rabbit bone marrow stromal cells. Information

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LANGUAGE: eng

NlmUniqueID: 376340

Characterization of growth and osteogenic differentiation of rabbit bone marrow stromal cells. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta1

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Characterization of growth and osteogenic differentiation of rabbit bone marrow stromal cells. Information

Substance Name: Osteopontin

Registry Number: 106441-73-0

Grant and Affiliation Information for Characterization of growth and osteogenic differentiation of rabbit bone marrow stromal cells.

AFFILIATION: Plastic Surgery Section, VA Greater LA Healthcare System, Los Angeles, California 90073, USA.

Country: United States

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MEDLINETA: J Surg Res

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