Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals.

Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals. Abstract Text:

S Kudo,M Onda,M Fukuda,

Glycophorins A (GPA) and B (GPB) are specifically expressed in human erythrocytes and express MN and Ss blood group antigens. While the GPB gene produces one transcript, the GPA gene produces three or four transcripts of different sizes. To understand the mechanisms of production of the different transcripts and erythroid-specific expression, we characterized the transcripts and the transcriptional regulatory regions of the glycophorin A gene. The transcriptional start site was determined by primer extension and S1 nuclease protection analysis, and it was found that all of the transcripts start at one major site. The nucleotide sequence of the newly isolated longest GPA cDNA revealed several polyadenylation signal sequences. To determine which polyadenylation signals are utilized, cDNA clones encoding GPA were isolated from a cDNA library of human erythroleukemia cell line K562 and the 3'-regions of the cDNAs were specifically amplified by the polymerase chain reaction. These results showed that five different polyadenylation signals of the GPA gene are utilized and the size and abundance of the transcripts are consistent with those detected by Northern blot analysis of K562 mRNAs. The 5'-flanking sequence was found to contain several binding motifs for the transcription factors, which were also found in other erythroid-specific genes. These motifs include the binding sites for the GATA-1 and NF-E2 erythroid-specific transcription factors, in addition to Sp1 transcription factor. The 5'-sequence from -750 to +36 relative to the transcriptional start site could confer only a low basal transcriptional activity in K562 cells and Friend erythroleukemia cells. This activity was significantly increased after Friend erythroleukemia cells were differentiated by dimethylsulfoxide. These results suggest that the 5' region regulates the glycophorin gene expression in erythroid-specific fashion.

Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals. Publishing Authors By Initials

S Kudo,M Onda,M Fukuda,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, messenger research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, messenger research

PUBMED ID PMID:

MEDLINE DATE:

Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of biochemistry

VOLUME: 116

Page Numbers: 183-92

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jul

YEAR: 1994

Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals. Keywords Mesh Terms:

KEYWORDS: RNA, Messenger

MESH TERMS: analysis

Chemical & Substance for Abstract: Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals. Information

Substance Name: Poly A

Registry Number: 24937-83-5

Grant and Affiliation Information for Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals.

AFFILIATION: La Jolla Cancer Research Foundation, Cancer Research Center, CA 92037.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER: L31863

Number Hits: 0

Characterization of glycophorin A transcripts: control by the common erythroid-specific promoter and alternative usage of different polyadenylation signals Related Publications

• A distinctive set of genes is upregulated during the inflammation-carcinoma sequence in mouse stomach infected by Helicobacter felis.
• AAV-PGIS gene transfer improves hypoxia-induced pulmonary hypertension in mice.
• Cell type-specific roles of carbohydrates in tumor metastasis.
• Cellular and molecular analysis of neural development of glycosyltransferase gene knockout mice.
• Changes in temporomandibular disc position during growth in young Japanese.
• Changes in the expression of cation-Cl- cotransporters, NKCC1 and KCC2, during cortical malformation induced by neonatal freeze-lesion.
• Characterization and host range determination of soybean super virulent Agrobacterium tumefaciens KAT23.
• Characterization of ligV essential for catabolism of vanillin by Sphingomonas paucimobilis SYK-6.
• Configuration of a chiral smectic-C film with a circular inclusion.
• Degradation of 3-O-methylgallate in Sphingomonas paucimobilis SYK-6 by pathways involving protocatechuate 4,5-dioxygenase.
• Diagnosing active inflammation in the SAPHO syndrome using 18FDG-PET/CT in suspected metastatic vertebral bone tumors.
• Expression cloning of cholesterol alpha-glucosyltransferase, a unique enzyme that can be inhibited by natural antibiotic gastric mucin O-glycans, from Helicobacter pylori.
• Expression of specific carbohydrates by transfection with carbohydrate modifying enzymes.
• Expression of vascular endothelial growth factor (VEGF) and its two receptors in diffusely infiltrating astrocytomas and relationship to proliferative activity of tumor cells.
• Flexible eye model for preoperative explanations.
• Intraoperative surgical clearance confirmation of tongue carcinomas using ultrasound.
• Leiomyomatosis peritonealis disseminata with malignant change in a man.
• Living radical polymerizations with germanium, tin, and phosphorus catalysts--reversible chain transfer catalyzed polymerizations (RTCPs).
• Matrix metalloproteinase 2: an important genetic marker for cholesteatomas.
• Polysialic acid-directed migration and differentiation of neural precursors are essential for mouse brain development.
• Prognostic significance of cyclin D1 and p16 in patients with intermediate-risk head and neck squamous cell carcinoma treated with docetaxel and concurrent radiotherapy.
• Roles of mucin-type O-glycans synthesized by core2beta1,6-N-acetylglucosaminyltransferase.
• Screening of peptide-displaying phage libraries to identify short peptides mimicking carbohydrates.
• Selective attention: psi performance in children with learning disabilities.
• Significant decrease in alpha1,3-linked fucose in association with increase in 6-sulfated N-acetylglucosamine in peripheral lymph node addressin of FucT-VII-deficient mice exhibiting diminished lymphocyte homing.
• Similarities of the neuronal circuit for the induction of fictive vomiting between ferrets and dogs.
• Stereotactic radiofrequency thermocoagulation for hypothalamic hamartoma with intractable gelastic seizures.
• TERE; a novel cis-element responsible for a coordinated expression of genes related to programmed cell death and secondary wall formation during differentiation of tracheary elements.
• Trophoblast cell activation by trophinin ligation is implicated in human embryo implantation.
• Usefulness of high-b-value diffusion-weighted imaging in acute cerebral infarction.