Characterization of genetically modified maize in weakly contaminated seed batches and identification of the origin of the adventitious contamination.

Characterization of genetically modified maize in weakly contaminated seed batches and identification of the origin of the adventitious contamination. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of genetically modified maize in weakly contaminated seed batches and identification of the origin of the adventitious contamination. Abstract Text:

Laetitia Petit, Pagny,Fabienne Baraige, Nignol,David Zhang,Patrick Fach,

So far, relatively few genetically modified plants (GMPs) have been planted in the European Union (EU). However, in France, seed batches weakly contaminated by unidentified GM materials have recently been detected among commercial maize seeds (14 seed batches positive out of 447 analyzed). We have developed a 3-step approach to precisely identify the genetic modifications detected in such maize seed batches. First, to isolate GMPs derived from the contaminated seed batches, 10 000 maize seeds of each batch were planted and screened by polymerase chain reaction (PCR) on 100-plant batches, then on 10-plant subbatches, and finally, plant by plant. In a second step, specific identification of the individual GMPs was performed. Finally, to determine the origin of the contamination, each individual GMP was analyzed by simple sequence repeat (SSR) markers. The results showed that all batches were contaminated by few GM seeds, having a GM content < 0.1%. Finally, 12 individual GMPs have been isolated from 17 plant pools that were tested positive either for P35-S and/or T-Nos. MON810 and T25 transformation events approved for cultivation in the EU were detected in 7 individual GMPs. The other seed batches were contaminated by genetically modified organisms (GMOs) that are not approved in the EU, including GA21 or the stacking MON810/T25. Presumable identification of T14 was also achieved following sequencing of 1 individual GMP. The data also showed that most of the seed batches were contaminated by several transformation events. Finally, analysis of SSR markers indicated that the contaminations were essentially due to cross-pollination in the seed production process.

Characterization of genetically modified maize in weakly contaminated seed batches and identification of the origin of the adventitious contamination. Publishing Authors By Initials

L Petit,G Pagny,F Baraige,AC Nignol,D Zhang,P Fach,

For similar plants: plant families and groups: angiosperms: poaceae: zea mays research abstracts see: plants: plant families and groups: angiosperms: poaceae: zea mays research

PUBMED ID PMID:

MEDLINE DATE:

Characterization of genetically modified maize in weakly contaminated seed batches and identification of the origin of the adventitious contamination. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of AOAC International

VOLUME: 90

Page Numbers: 1098-106

Journal Abbreviation:

ISSN: 1060-3271

DAY: 29

MONTH: 11

YEAR: 2007

Characterization of genetically modified maize in weakly contaminated seed batches and identification of the origin of the adventitious contamination. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9215446

Characterization of genetically modified maize in weakly contaminated seed batches and identification of the origin of the adventitious contamination. Keywords Mesh Terms:

KEYWORDS: Zea mays

MESH TERMS: metabolism

Chemical & Substance for Abstract: Characterization of genetically modified maize in weakly contaminated seed batches and identification of the origin of the adventitious contamination. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Characterization of genetically modified maize in weakly contaminated seed batches and identification of the origin of the adventitious contamination.

AFFILIATION: Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes et de Recherches sur la Qualité des Aliments et sur les Procédés Agro-Alimentaires, Unité EBA, 23 Avenue du Général De Gaulle, 94700 Maisons-Alfort, France.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J AOAC Int

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization of genetically modified maize in weakly contaminated seed batches and identification of the origin of the adventitious contamination Related Publications

• Characterization of genetically modified maize in weakly contaminated seed batches and identification of the origin of the adventitious contamination.
• Development of a 5'-nuclease PCR assay for the identification of Escherichia coli strains expressing the flagellar antigen H21 and their detection in food after enrichment.