Characterization of genetic polymorphism of novel MHC B-LB II alleles in Chinese indigenous chickens.

Characterization of genetic polymorphism of novel MHC B-LB II alleles in Chinese indigenous chickens. Research Abstract Details 

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Characterization of genetic polymorphism of novel MHC B-LB II alleles in Chinese indigenous chickens. Abstract Text:

Rifu Xu,Kui Li,Guohong Chen,Hui Xu,Bayangzong Qiang,Changchun Li,Bang Liu,

Genetic polymorphism of the major histocompatibility complex (MHC) B-LB II gene was studied by amplification of exon 2 using PCR, followed by cloning and DNA sequencing in eight indigenous Chinese chicken populations. To reveal the genetic variation of the B-LB II gene, 37 types of patterns detected by PCR-SSCP were investigated first, which would be used to screen novel B-LB II sequences within the breeds. The types of PCR-SSCP patterns and final sequencing allowed for the identification of 31 novel MHC B-LB II alleles from 30 unrelated individuals of Chinese chickens that were sampled. These are the first designators for the alleles of chicken MHC B-LB II gene based on the rule of assignment for novel mammalian alleles. Sequence alignment of the 31 B-LB II alleles revealed a total of 68 variable sites in the fragment of exon 2, of which 51 parsimony informative and 17 singleton variable sites were observed. Among the polymorphic sites, the nucleotide substitutions in the first and second positions of the codons accounted for 36.76% and 35.29%, respectively. The sequence similarities between the alleles were estimated to be 90.6%-99.5%. The relative frequencies of synonymous and nonsynonymous nucleotide substitutions within the region were 2.92%+/-0.94% and 14.64%+/-2.67%, respectively. These results indicated that the genetic variation within exon 2 appeared to have largely arisen by gene recombination and balancing selection. Alignment of the deduced amino acid sequences of the beta1 domain coded by exon 2 revealed 6 synonymous mutations and 27 nonsynonymous substitutions at the 33 disparate sites. In particular, the nonsynonymous substitutions at the putative peptide-binding sites are considered to be associated with immunological specificity of MHC B-LB II molecule in Chinese native chickens. These results can provide a molecular biological basis for the study of disease resistance in chicken breeding.

Characterization of genetic polymorphism of novel MHC B-LB II alleles in Chinese indigenous chickens. Publishing Authors By Initials

R Xu,K Li,G Chen,H Xu,B Qiang,C Li,B Liu,

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Characterization of genetic polymorphism of novel MHC B-LB II alleles in Chinese indigenous chickens. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of genetics and genomics = Yi chuan xue ba

VOLUME: 34

Page Numbers: 109-18

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ISSN: 1673-8527

DAY: 1

MONTH: Feb

YEAR: 2007

Characterization of genetic polymorphism of novel MHC B-LB II alleles in Chinese indigenous chickens. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Characterization of genetic polymorphism of novel MHC B-LB II alleles in Chinese indigenous chickens.

AFFILIATION: College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Country: China

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MEDLINETA: J Genet Genomics

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