Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis.

Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis. Research Abstract Details 

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Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis. Abstract Text:

Yoshinobu Katoh,Hirokazu Kouguchi,Jun Matsumoto,Akiko Goto,Tomohiro Suzuki,Yuzaburo Oku,Kinpei Yagi,Yoshinobu Katoh,Hirokazu Kouguchi,Jun Matsumoto,Akiko Goto,Tomohiro Suzuki,Yuzaburo Oku,Kinpei Yagi,

A cDNA library based on mRNA from adult worms of Echinococcus multilocularis was constructed. One cDNA clone, emY162, was isolated from this cDNA library. The putative protein from emY162 cDNA consists of 153 amino acids and has a predicted molecular weight of 17.0 kDa. The amino acid sequences of EMY162 are predicted to be a hydrophobic N-terminus conserving a secretory signal, and a hydrophobic C-terminus encoding a transmembrane domain or glycosyl-phosphatylinositol membrane anchor, and to have single fibronectin type III-like domain. In addition, it was shown that the emY162 gene (1738 bp) in the E. multilocularis genome DNA consists of three exons and two introns, and that emY162 is expressed in all four stages (protoscoleces, cultured metacestodes, immature adult worms and mature adult worms). Moreover, immunity to recombinant EMY162, which comprises the fibronectin type III-like domain on the EMY162 protein, was examined. Immune responses to the recombinant EMY162 were studied by using serum from dogs infected with E. multilocularis. Strong IgG immune responses were detected in Western blots.

Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis. Publishing Authors By Initials

Y Katoh,H Kouguchi,J Matsumoto,A Goto,T Suzuki,Y Oku,K Yagi,Y Katoh,H Kouguchi,J Matsumoto,A Goto,T Suzuki,Y Oku,K Yagi,

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Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochimica et biophysica acta

VOLUME: 1780

Page Numbers: 1-6

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 18

MONTH: 09

YEAR: 2007

Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis. Information

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LANGUAGE: eng

NlmUniqueID: 217513

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Grant and Affiliation Information for Characterization of emY162 encoding an immunogenic protein cloned from an adult worm-specific cDNA library of Echinococcus multilocularis.

AFFILIATION: Department of Biological Science, Center for Infectious Disease Prevention, Hokkaido Institute of Public Health, Sapporo, Hokkaido 060-0819, Japan.

Country: Netherlands

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MEDLINETA: Biochim Biophys Acta

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