Characterization of Deltoid, a chimeric protein containing the oligomerization site of hepatitis delta antigen.

Characterization of Deltoid, a chimeric protein containing the oligomerization site of hepatitis delta antigen. Research Abstract Details 

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Characterization of Deltoid, a chimeric protein containing the oligomerization site of hepatitis delta antigen. Abstract Text:

Matthew J Saderholm,Stanley M Lemon,Bruce W Erickson,Matthew J Saderholm,Stanley M Lemon,Bruce W Erickson,

Both forms of the hepatitis delta antigen (HDAg) encoded by hepatitis delta virus are active only as oligomers. Previous studies showed that quadrin, a synthetic 50-residue peptide containing residues 12-60 from the N-terminus of HDAg, interferes with HDAg oligomerization, forms an alpha-helical coiled coil in solution, and forms a novel square octamer in the crystal consisting of four antiparallel coiled-coil dimers joined at the corners by hydrophobic binding of oligomerization sites located at each end of the dimers. We designed and synthesized deltoid (CH3CO-[Cys23]HDAg-(12-27)-seryl-tRNA synthetae-(59-65)-[Cys42]HDAg-(34-60)-Tyr-NH2), a chimeric protein that structurally resembles one end of the quadrin dimer and contains a single oligomerization site. The 51-residue chain of deltoid contains a seven-residue alpha-hairpin loop in place of the remainder of the quadrin dimer plus Cys12 and Cys31 for forming an intrachain disulfide bridge. Reduced, unbridged deltoid (Tm=61 degrees C, DeltaG(H2O)=-1.7 kcal mol(-1)) was less stable to denaturation by heat or guanidine HCl than oxidized, intrachain disulfide-bridged deltoid (Tm>80 degrees C, DeltaG(H2O)=-2.6 kcal mol(-1)). Each form is an alpha-helical dimer that reversibly dissociates into two monomers (Kd=80 microM).

Characterization of Deltoid, a chimeric protein containing the oligomerization site of hepatitis delta antigen. Publishing Authors By Initials

MJ Saderholm,SM Lemon,BW Erickson,MJ Saderholm,SM Lemon,BW Erickson,

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Characterization of Deltoid, a chimeric protein containing the oligomerization site of hepatitis delta antigen. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biopolymers

VOLUME: 88

Page Numbers: 764-73

Journal Abbreviation: Biopolymers

ISSN: 0006-3525

DAY: 12

MONTH: 09

YEAR: 2007

Characterization of Deltoid, a chimeric protein containing the oligomerization site of hepatitis delta antigen. Information

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LANGUAGE: eng

NlmUniqueID: 372525

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Grant and Affiliation Information for Characterization of Deltoid, a chimeric protein containing the oligomerization site of hepatitis delta antigen.

AFFILIATION: Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. matthew_saderholm@berea.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM42031

ACRONYM: GM

MEDLINETA: Biopolymers

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