Characterization of cytochrome b(5) in the ascidian Polyandrocarpa misakiensis and budding-specific expression.

Characterization of cytochrome b(5) in the ascidian Polyandrocarpa misakiensis and budding-specific expression. Research Abstract Details 

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Characterization of cytochrome b(5) in the ascidian Polyandrocarpa misakiensis and budding-specific expression. Abstract Text:

T Yubisui,F Takahashi,T Takabayashi,S Fujiwara,K Kawamura,

A cDNA for cytochrome b(5) was cloned from a cDNA library of buds of the ascidian, Polyandrocarpa misakiensis, by a hybridization method involving a digoxigenin-labeled cDNA probe of human soluble cytochrome b(5). The nucleotide sequence of the cDNA for the ascidian cytochrome b(5) (Pmb5) consisted of about 1,800 base pairs including 5'- and 3'-noncoding regions, and a coding sequence of 405 base pairs. The amino acid sequence of 135 residues deduced from the coding nucleotide sequence exhibited 54% identity and 76% similarity to chicken cytochrome b(5). A highly conserved amino acid sequence was observed in the amino-terminal domain of 96 residues containing two heme-binding histidine residues. The putative soluble form of the recombinant Pmb5 expressed in Escherichia coli was purified to homogeneity by column chromatographies on an anion-exchanger and gel filtration. The purified Pmb5 showed the typical absorption spectrum of cytochrome b(5) with an asymmetric peak at 556 nm and a shoulder at 560 nm upon reduction with NADH and NADH-cytochrome b(5) reductase. The low temperature spectrum of the dithionite-reduced form of the protein contained the split peaks at 551 and 555 nm, this spectrum being very similar to that of mammalian liver cytochrome b(5). Expression of Pmb5 in the ascidian was examined immunohistochemically with a monoclonal antibody against the Pmb5. Apparently high level expression of Pmb5 was found in the developing buds, but the levels of cytochrome b(5) in the parents and juvenile adults were very low. This is the first report on the characterization of Pmb5, and the increased expression of Pmb5 in the ascidian.

Characterization of cytochrome b(5) in the ascidian Polyandrocarpa misakiensis and budding-specific expression. Publishing Authors By Initials

T Yubisui,F Takahashi,T Takabayashi,S Fujiwara,K Kawamura,

For similar animals: chordata: chordata, nonvertebrate: urochordata research abstracts see: animals: chordata: chordata, nonvertebrate: urochordata research

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Characterization of cytochrome b(5) in the ascidian Polyandrocarpa misakiensis and budding-specific expression. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 129

Page Numbers: 709-16

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 2001

Characterization of cytochrome b(5) in the ascidian Polyandrocarpa misakiensis and budding-specific expression. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Characterization of cytochrome b(5) in the ascidian Polyandrocarpa misakiensis and budding-specific expression. Keywords Mesh Terms:

KEYWORDS: Urochordata

MESH TERMS: enzymology

Chemical & Substance for Abstract: Characterization of cytochrome b(5) in the ascidian Polyandrocarpa misakiensis and budding-specific expression. Information

Substance Name: Cytochromes b5

Registry Number: 9035-39-6

Grant and Affiliation Information for Characterization of cytochrome b(5) in the ascidian Polyandrocarpa misakiensis and budding-specific expression.

AFFILIATION: Laboratory of Cellular and Molecular Biotechnology, Faculty of Science, Kochi University, Akebono-cho, Kochi 780-8520, Japan. tyubi@cc.kochi-u.ac.jp

Country: Japan

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MEDLINETA: J Biochem

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ACCESSION NUMBER: AB042613

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