Characterization of cis-autoproteolysis of polycystin-1, the product of human polycystic kidney disease 1 gene.

Characterization of cis-autoproteolysis of polycystin-1, the product of human polycystic kidney disease 1 gene. Research Abstract Details 

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Characterization of cis-autoproteolysis of polycystin-1, the product of human polycystic kidney disease 1 gene. Abstract Text:

Wen Wei,Karl Hackmann,Hangxue Xu,Gregory Germino,Feng Qian,

Polycystin-1 (PC1), the PKD1 gene product, plays a critical role in renal tubule diameter control and disruption of its function causes cyst formation in human autosomal dominant polycystic kidney disease. Recent evidence shows that PC1 undergoes cleavage at the juxtamembrane G protein-coupled receptor proteolytic site (GPS), a process likely to be essential for its biological activity. Here we further characterized the proteolytic cleavage of PC1 at the GPS domain. We determined the actual cleavage site to be between leucine and threonine of the tripeptide HLT(3049) of human PC1. Cleavage occurs in the early intracellular secretory pathway and requires initial N-glycan attachment but not its subsequent trimming. We provide evidence that the cleavage occurs via a cis-autoproteolytic mechanism involving an ester intermediate as shown for Ntn hydrolases and EMR2.

Characterization of cis-autoproteolysis of polycystin-1, the product of human polycystic kidney disease 1 gene. Publishing Authors By Initials

W Wei,K Hackmann,H Xu,G Germino,F Qian,

For similar proteins: carrier proteins: membrane transport proteins: ion channels: calcium channels: trpp cation channels research abstracts see: proteins: carrier proteins: membrane transport proteins: ion channels: calcium channels: trpp cation channels research

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Characterization of cis-autoproteolysis of polycystin-1, the product of human polycystic kidney disease 1 gene. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 21729-37

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 24

MONTH: 05

YEAR: 2007

Characterization of cis-autoproteolysis of polycystin-1, the product of human polycystic kidney disease 1 gene. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

Characterization of cis-autoproteolysis of polycystin-1, the product of human polycystic kidney disease 1 gene. Keywords Mesh Terms:

KEYWORDS: TRPP Cation Channels

MESH TERMS: metabolism

Chemical & Substance for Abstract: Characterization of cis-autoproteolysis of polycystin-1, the product of human polycystic kidney disease 1 gene. Information

Substance Name: G-protein coupled receptor OR3

Registry Number: 149024-22-6

Grant and Affiliation Information for Characterization of cis-autoproteolysis of polycystin-1, the product of human polycystic kidney disease 1 gene.

AFFILIATION: Division of Nephrology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: R01 DK062199

ACRONYM: DK

MEDLINETA: J Biol Chem

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