Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk.

Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk. Abstract Text:

L T Pallesen,L R L Pedersen,T E Petersen,J T Rasmussen,

The present work reports the characterization of carbohydrate structures and the distribution of the newly identified mucin MUC15, a highly glycosylated protein associated with the bovine milk fat globule membrane (MFGM). Distribution of MUC15 was investigated in various fractions of bovine milk by densitometric scanning of Western blots. In raw milk, MUC15 was shown to constitute 0.08% (wt) of the protein and approximately 1.5% (wt) of the MFGM-associated proteins. Surprisingly, this study showed that in addition to the fat-containing fractions, such as MFGM and buttermilk, MUC15 was present in nonfat-containing fractions as well, such as skim milk and whey. Compositional and structural studies of the carbohydrates of bovine milk MUC15 showed that the glycans are composed of fucose, galactose, mannose, N-acetylgalactosamine, N-acetylglycosamine, and sialic acid. The carbohydrate was shown to constitute 65% of the total molecular weight, and the molar ratios of the individual sugars to protein of the O-linked glycans were determined. The glycan structures of MUC15 were further studied by enzymatic deglycosylation experiments using different endo- and exoglycosidases as well as a panel of lectins. The N-linked glycans were shown to contain mainly hybrid-type N-glycans. In addition, the N-glycans were shown to be sialylated and contain terminal poly-lactosamine structures. The O-linked glycans were found to constitute some unsubstituted Core-1 structures and a substantial number of sialylated Core-1 O-linked glycans. By comparing the results of peanut agglutinin lectin binding, enzymatic deglycosylation, and monosaccharide composition analysis, we concluded that bovine MUC15 also contains more complex O-glycans containing high amounts N-acetylglucosamine residues. Furthermore, a small subset of the O-linked glycans is decorated with lactosamine on their terminal ends.

Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk. Publishing Authors By Initials

LT Pallesen,LR Pedersen,TE Petersen,JT Rasmussen,

For similar carbohydrates: polysaccharides research abstracts see: carbohydrates: polysaccharides research

PUBMED ID PMID:

MEDLINE DATE:

Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of dairy science

VOLUME: 90

Page Numbers: 3143-52

Journal Abbreviation: J. Dairy Sci.

ISSN: 1525-3198

DAY: 27

MONTH: Jul

YEAR: 2007

Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985126

Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk. Keywords Mesh Terms:

KEYWORDS: Polysaccharides

MESH TERMS: chemistry

Chemical & Substance for Abstract: Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk. Information

Substance Name: Peptide-N4-(N-acetyl-beta-glucosaminyl)

Registry Number: EC 3.5.1.52

Grant and Affiliation Information for Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk.

AFFILIATION: Protein Chemistry Laboratory, Department of Molecular Biology, University of Aarhus, 8000 Aarhus C, Denmark.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Dairy Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk Related Publications

• A Chronic Increase in Energy Expenditure Inhibits S6K1 and Reduces IRS-1 Serine Phosphorylation in Muscle: 540: May 30 10:30 AM - 10:45 AM.
• Association between intelligence and coronary heart disease mortality: a population-based cohort study of 682 361 Swedish men.
• Association of maternal obesity before conception with poor lactation performance.
• Blood Flow Restriction during Low-Intensity Resistance Exercise Increases Muscle Protein Synthesis through the mTOR Signaling Pathway: 666: May 31 8:00 AM 8:15 AM.
• Characterization of carbohydrate structures of bovine MUC15 and distribution of the mucin in bovine milk.
• Comparative proteomics of cell division mutants and wild-type of Synechococcus sp. strain PCC 7942.
• Cranial implant design using augmented reality immersive system.
• Distinct distribution of autosomal dominant spinocerebellar ataxia in the Mexican population.
• Dysregulation of muscle fatty acid metabolism in type 2 diabetes is independent of malonyl-CoA.
• Experiences with carrier-mediated transport in liquid-phase microextraction.
• Extraction across supported liquid membranes by use of electrical fields.
• Genome-wide reprogramming in hybrids of somatic cells and embryonic stem cells.
• Haptic rendering of volumetric data for cranial implant modeling.
• Hysterectomy is associated with stress incontinence in women who previously had a transcervical endometrial resection.
• Impulse control disorders in adults with obsessive compulsive disorder.
• Inhibitory activities of bovine macromolecular whey proteins on rotavirus infections in vitro and in vivo.
• Inhibitory effects of human and bovine milk constituents on rotavirus infections.
• Late-onset obsessive compulsive disorder: clinical characteristics and psychiatric comorbidity.
• Mechanisms controlling death, survival and proliferation in a model unicellular eukaryote Tetrahymena thermophila.
• Microemulsion electrokinetic chromatography in suppressed electroosmotic flow environment. Separation of fat-soluble vitamins.
• Muscle Protein Synthesis is Enhanced Post-Resistance Exercise by Nutrient Stimulation of the mTOR Signaling Pathway: 873: June 1 1:30 PM - 1:45 PM.
• Olanzapine-induced weight gain and increased visceral adiposity is blocked by melatonin replacement therapy in rats.
• Opening rates of DNA hairpins: experiment and model.
• Postoperative cognitive dysfunction in patients with preoperative cognitive impairment: which domains are most vulnerable?
• Sexual obsessions and clinical correlates in adults with obsessive-compulsive disorder.
• Simulation of eye disease in virtual reality.
• Small RNAs controlling outer membrane porins.
• Stress distributions in diblock copolymers.
• Taboo thoughts and doubt/checking: a refinement of the factor structure for obsessive-compulsive disorder symptoms.
• The brown longitudinal obsessive compulsive study: treatments received and patient impressions of improvement.