Characterization of antibody to 2'-5' linked oligoadenylic acid.

Characterization of antibody to 2'-5' linked oligoadenylic acid. Research Abstract Details 

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Characterization of antibody to 2'-5' linked oligoadenylic acid. Abstract Text:

H Sawai,T Shinomiya,

Antibody to 2'-5' linked triadenylate, A2'p5'A2'p5'A, was prepared by injecting bovine serum albumin conjugated with A2'p5'A2'p5'A into rabbits. The [8-14C]-A2'p5'A2'p5'A used in the study was synthesized by nonenzymatic template-directed condensation of [8-14C]AMP and 5'-phosphorimidazolide of A2'p5'A on poly U. The specificity of the antibody was determined by the inhibition of the binding of the radioisotope probe to the antibody by a competitor using the ammonium sulfate precipitation method. The cross reactivity of a number of modified oligonucleotides has been evaluated in order to gain information on the structural feature involved in the binding to the antibody. The adenine moiety and 2'-5' internucleotide linkage are crucial regions for the binding. The antibody reacted not only on A2'p5'A2'p5'A and A2'p5'A2'p5'A2'p5'A, but also on a cordycepin analogue of the trimer, (3'dA)2'p5'(3'dA)2'p5'(3'dA), significantly. Considerable cross reaction was observed with oligoadenylates bearing both 2'-5' and 3'-5' internucleotide linkages, while there was little cross reaction with 3'-5' linked oligoadenylates. The antibody showed very low or no affinity for adenosine, adenine mononucleotides, other nucleotides and other 2'-5' linked trinucleotides. The cross reactivity of the modified oligonucleotides is indicated to have relation to the biological activity of the corresponding 5'-phosphorylated oligonucleotides, so far reported. The antibody was further purified by affinity chromatography using RNA-Sepharose and AMP-Sepharose giving high specificity.

Characterization of antibody to 2'-5' linked oligoadenylic acid. Publishing Authors By Initials

H Sawai,T Shinomiya,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: molecular conformation: protein conformation research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: molecular conformation: protein conformation research

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Characterization of antibody to 2'-5' linked oligoadenylic acid. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 92

Page Numbers: 1723-30

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1982

Characterization of antibody to 2'-5' linked oligoadenylic acid. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Characterization of antibody to 2'-5' linked oligoadenylic acid. Keywords Mesh Terms:

KEYWORDS: Protein Conformation

MESH TERMS: immunology

Chemical & Substance for Abstract: Characterization of antibody to 2'-5' linked oligoadenylic acid. Information

Substance Name: 2',5'-oligoadenylate

Registry Number: 61172-40-5

Grant and Affiliation Information for Characterization of antibody to 2'-5' linked oligoadenylic acid.

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Country: JAPAN

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MEDLINETA: J Biochem

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