Characterization of alternatively spliced isoforms of the type I interleukin-1 receptor on iNOS induction in rat hepatocytes.

Characterization of alternatively spliced isoforms of the type I interleukin-1 receptor on iNOS induction in rat hepatocytes. Research Abstract Details 

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Characterization of alternatively spliced isoforms of the type I interleukin-1 receptor on iNOS induction in rat hepatocytes. Abstract Text:

Masanori Yamada,Mikio Nishizawa,Richi Nakatake,Kozo Habara,Hideyuki Yoshida,Takashi Ozaki,Kosuke Matsui,Yoshinori Hamada,Yasuo Kamiyama,Seiji Ito,Tadayoshi Okumura,

In animal models of liver injury, proinflammatory cytokines are implicated in inducing iNOS, which is followed by the production of NO in hepatocytes. Previously we have reported that the up-regulation of type I IL-1 receptor (IL-1RI) is required for the transcriptional activation of iNOS gene, in concert with the activation of transcription factor NF-kappaB. In this study, we found three alternatively spliced isoforms of IL-1RI in primary cultured rat hepatocytes: two (long and short) membrane-bound and one soluble IL-1RI. Interleukin (IL)-1beta markedly augmented the mRNA levels of long and short IL-1RI with time, but was less effective for soluble IL-1RI. Two membrane-bound IL-1RI were localized in the intracellular fraction, whereas soluble IL-1RI was released into the culture medium. Cotransfection experiments with iNOS promoter-luciferase constructs revealed that the overexpression of long and short IL-1RI, but not soluble IL-1RI, significantly increased the transactivation of iNOS promoter and the stabilization of its mRNA. In contrast, the addition of conditioned medium containing soluble IL-1RI reduced the induction of iNOS and NO production stimulated by IL-1beta. These results further suggest that the enhancement of IL-1RI isoforms may contribute to the regulation of iNOS induction in hepatocytes.

Characterization of alternatively spliced isoforms of the type I interleukin-1 receptor on iNOS induction in rat hepatocytes. Publishing Authors By Initials

M Yamada,M Nishizawa,R Nakatake,K Habara,H Yoshida,T Ozaki,K Matsui,Y Hamada,Y Kamiyama,S Ito,T Okumura,

For similar genetic processes: gene expression regulation: trans-activation (genetics) research abstracts see: genetic processes: gene expression regulation: trans-activation (genetics) research

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Characterization of alternatively spliced isoforms of the type I interleukin-1 receptor on iNOS induction in rat hepatocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nitric oxide : biology and chemistry / official jo

VOLUME: 17

Page Numbers: 98-105

Journal Abbreviation: Nitric Oxide

ISSN: 1089-8603

DAY: 1

MONTH: 07

YEAR: 2007

Characterization of alternatively spliced isoforms of the type I interleukin-1 receptor on iNOS induction in rat hepatocytes. Information

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LANGUAGE: eng

NlmUniqueID: 9709307

Characterization of alternatively spliced isoforms of the type I interleukin-1 receptor on iNOS induction in rat hepatocytes. Keywords Mesh Terms:

KEYWORDS: Trans-Activation (Genetics)

MESH TERMS: physiology

Chemical & Substance for Abstract: Characterization of alternatively spliced isoforms of the type I interleukin-1 receptor on iNOS induction in rat hepatocytes. Information

Substance Name: Nitric Oxide Synthase Type II

Registry Number: EC 1.14.13.39

Grant and Affiliation Information for Characterization of alternatively spliced isoforms of the type I interleukin-1 receptor on iNOS induction in rat hepatocytes.

AFFILIATION: Department of Surgery, Kansai Medical University, Moriguchi, Osaka 570-8506, Japan.

Country: United States

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MEDLINETA: Nitric Oxide

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