Characterization of Agrobacterium tumefaciens DNA ligases C and D.

Characterization of Agrobacterium tumefaciens DNA ligases C and D. Research Abstract Details 

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Characterization of Agrobacterium tumefaciens DNA ligases C and D. Abstract Text:

Hui Zhu,Stewart Shuman,

Agrobacterium tumefaciens encodes a single NAD+-dependent DNA ligase and six putative ATP-dependent ligases. Two of the ligases are homologs of LigD, a bacterial enzyme that catalyzes end-healing and end-sealing steps during nonhomologous end joining (NHEJ). Agrobacterium LigD1 and AtuLigD2 are composed of a central ligase domain fused to a C-terminal polymerase-like (POL) domain and an N-terminal 3'-phosphoesterase (PE) module. Both LigD proteins seal DNA nicks, albeit inefficiently. The LigD2 POL domain adds ribonucleotides or deoxyribonucleotides to a DNA primer-template, with rNTPs being the preferred substrates. The LigD1 POL domain has no detectable polymerase activity. The PE domains catalyze metal-dependent phosphodiesterase and phosphomonoesterase reactions at a primer-template with a 3'-terminal diribonucleotide to yield a primer-template with a monoribonucleotide 3'-OH end. The PE domains also have a 3'-phosphatase activity on an all-DNA primer-template that yields a 3'-OH DNA end. Agrobacterium ligases C2 and C3 are composed of a minimal ligase core domain, analogous to Mycobacterium LigC (another NHEJ ligase), and they display feeble nick-sealing activity. Ligation at DNA double-strand breaks in vitro by LigD2, LigC2 and LigC3 is stimulated by bacterial Ku, consistent with their proposed function in NHEJ.

Characterization of Agrobacterium tumefaciens DNA ligases C and D. Publishing Authors By Initials

H Zhu,S Shuman,

For similar bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: rhizobiaceae: rhizobium: rhizobium radiobacter research abstracts see: bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: rhizobiaceae: rhizobium: rhizobium radiobacter research

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Characterization of Agrobacterium tumefaciens DNA ligases C and D. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nucleic acids research

VOLUME: 35

Page Numbers: 3631-45

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 8

MONTH: 05

YEAR: 2007

Characterization of Agrobacterium tumefaciens DNA ligases C and D. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 411011

Characterization of Agrobacterium tumefaciens DNA ligases C and D. Keywords Mesh Terms:

KEYWORDS: Rhizobium radiobacter

MESH TERMS: enzymology

Chemical & Substance for Abstract: Characterization of Agrobacterium tumefaciens DNA ligases C and D. Information

Substance Name: DNA ligase (ATP)

Registry Number: EC 6.5.1.1

Grant and Affiliation Information for Characterization of Agrobacterium tumefaciens DNA ligases C and D.

AFFILIATION: Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10021, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM63611

ACRONYM: GM

MEDLINETA: Nucleic Acids Res

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