Characterization of acetohexamide reductases purified from rabbit liver, kidney, and heart: structural requirements for substrates and inhibitors.

Characterization of acetohexamide reductases purified from rabbit liver, kidney, and heart: structural requirements for substrates and inhibitors. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization of acetohexamide reductases purified from rabbit liver, kidney, and heart: structural requirements for substrates and inhibitors. Abstract Text:

Y Imamura,T Koga,T Migita,A Ryu,M Otagiri,M Nozawa,H Akita,

The structural requirements of acetohexamide reductases purified from rabbit liver, kidney, and heart for substrates and inhibitors were examined. Acetohexamide, an oral antidiabetic drug with a ketone group, and analogs of it with various alkyl groups instead of the cyclohexyl group were used as substrates for these three enzymes. The results obtained as to substrate specificity suggested that the nature of the substrate-binding region of the heart enzyme is markedly different from those of the substrate-binding regions of the liver and kidney enzymes. Tolbutamide, which has no ketone group within its chemical structure, strongly inhibited the heart enzyme, whereas it had little ability to inhibit the liver or kidney enzyme. The inhibition of the heart enzyme by tolbutamide was competitive with respect to acetohexamide and uncompetitive with respect to NADPH. Furthermore, tolbutamide analogs with n-pentyl and n-hexyl groups instead of the n-butyl group exhibited very pronounced inhibition of only the heart enzyme. Therefore, it is reasonable to postulate that the heart enzyme, unlike the liver and kidney ones, has a cleft of a strongly hydrophobic nature near its substrate-binding region, and that this hydrophobic cleft plays a critical role in the interaction of the heart enzyme with the cyclohexyl group of acetohexamide.

Characterization of acetohexamide reductases purified from rabbit liver, kidney, and heart: structural requirements for substrates and inhibitors. Publishing Authors By Initials

Y Imamura,T Koga,T Migita,A Ryu,M Otagiri,M Nozawa,H Akita,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: triazines research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: triazines research

PUBMED ID PMID:

MEDLINE DATE:

Characterization of acetohexamide reductases purified from rabbit liver, kidney, and heart: structural requirements for substrates and inhibitors. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 121

Page Numbers: 705-10

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 1997

Characterization of acetohexamide reductases purified from rabbit liver, kidney, and heart: structural requirements for substrates and inhibitors. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Characterization of acetohexamide reductases purified from rabbit liver, kidney, and heart: structural requirements for substrates and inhibitors. Keywords Mesh Terms:

KEYWORDS: Triazines

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Characterization of acetohexamide reductases purified from rabbit liver, kidney, and heart: structural requirements for substrates and inhibitors. Information

Substance Name: acetohexamide reductase

Registry Number: EC 1.1.1.-

Grant and Affiliation Information for Characterization of acetohexamide reductases purified from rabbit liver, kidney, and heart: structural requirements for substrates and inhibitors.

AFFILIATION: Faculty of Pharmaceutical Sciences, Kumamoto University, Oe-honmachi.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization of acetohexamide reductases purified from rabbit liver, kidney, and heart: structural requirements for substrates and inhibitors Related Publications

• Bilateral Common Carotid Artery Aneurysm in Takayasu's Arteritis.
• Bone morphogenetic protein-2 regulates proliferation of human mesenchymal stem cells.
• Characterization of acetohexamide reductases purified from rabbit liver, kidney, and heart: structural requirements for substrates and inhibitors.
• Chemoenzymatic Synthesis of (+)-alpha-Polypodatetraene and Methyl (5R,10R,13R)-Labda-8-en-15-oate.
• Ciliary ultrastructure in two sisters with Kartagener's syndrome.
• Concise syntheses of coronarin a, coronarin e, austrochaparol and pacovatinin a.
• Concise syntheses of cystothiazoles A, C, D, and melithiazol B.
• Developmental processes and ectodermal contribution to the anal canal in mice.
• Humeral insertion of the supraspinatus and infraspinatus. New anatomical findings regarding the footprint of the rotator cuff.
• Ludloff's medial approach for open reduction of congenital dislocation of the hip. A 20-year follow-up.
• Predicting early coronary artery bypass graft failure by intraoperative transit time flow measurement.
• Process Development and Large-Scale Synthesis of NK(1) Antagonist.
• Protein kinase C-epsilon (PKC-epsilon): its unique structure and function.
• Purification and catalytic properties of a novel acetohexamide-reducing enzyme from rabbit heart.
• STUDIES ON THE ADENOSINE TRIPHOSPHATASE ACTIVITY OF VESICULAR RELAXING FACTOR.
• Saccular true aneurysm of the ascending aorta 19 years after aortic cannulation: report of a case.
• Syntheses of novel di- and trinucleating ligands having a triethylbenzene core with N,N-bidentate tethers: their complexation toward Pd and Rh organometallic fragments.
• The left inferior phrenic artery arising from left hepatic artery or left gastric artery: radiological and anatomical correlation in clinical cases and cadaver dissection.
• Three sets of translocation intermediates are formed during the early stage of protein import into chloroplasts.
• Total arch replacement for a distal arch aneurysm with aberrant right subclavian artery.
• [A method to instruct the student in case study of nursing]
• [A method to instruct the students in case studies of nursing]
• [A rare complication of catheter balloon valvuloplasty of pulmonary stenosis--a case report]
• [Apical vent of the left ventricle: is it necessary or not? (author's transl)]
• [Case study of the month: Down's syndrome. On adaptability of a child with Down's syndrome to group activities--nursing assistance relating to child development]
• [Hypothermia and potassium: significance in myocardial protection (author's transl)]
• [Microscopical studies of urinary calculi with a polarizing microscope.]
• [Porcine xenograft valve: a clinical study (author's transl)]
• [Potassium-induced multi dose cold blood cardioplegia: a clinical study (author's transl)]
• [Potassium-induced multidose cold blood cardioplegia: a clinical study (author's transl)]