Characterization of a spliced exon product of herpes simplex type-1 latency-associated transcript in productively infected cells.

Characterization of a spliced exon product of herpes simplex type-1 latency-associated transcript in productively infected cells. Research Abstract Details 

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Characterization of a spliced exon product of herpes simplex type-1 latency-associated transcript in productively infected cells. Abstract Text:

Wen Kang,Ruma Mukerjee,Jared J Gartner,Artemis G Hatzigeorgiou,Rozanne M Sandri-Goldin,Nigel W Fraser,

The latency-associated transcripts (LATs) of herpes simplex virus type-1 (HSV-1) are the only viral RNAs accumulating during latent infections in the sensory ganglia of the peripheral nervous system. The major form of LAT that accumulates in latently infected neurons is a 2 kb intron, spliced from a much less abundant 8.3 primary transcript. The spliced exon mRNA has been hard to detect. However, in this study, we have examined the spliced exon RNA in productively infected cells using ribonuclease protection (RPA), and quantitative RT-PCR (q-PCR) assays. We were able to detect the LAT exon RNA in productively infected SY5Y cells (a human neuronal cell line). The level of the LAT exon RNA was found to be approximately 5% that of the 2 kb intron RNA and thus is likely to be relatively unstable. Quantitative RT-PCR (q-PCR) assays were used to examine the LAT exon RNA and its properties. They confirmed that the LAT exon mRNA is present at a very low level in productively infected cells, compared to the levels of other viral transcripts. Furthermore, experiments showed that the LAT exon mRNA is expressed as a true late gene, and appears to be polyadenylated. In SY5Y cells, in contrast to most late viral transcripts, the LAT exon RNA was found to be mainly nuclear localized during the late stage of a productive infection. Interestingly, more LAT exon RNA was found in the cytoplasm in differentiated compared to undifferentiated SY5Y cells, suggesting the nucleocytoplasmic distribution of the LAT exon RNA and its related function may be influenced by the differentiation state of cells.

Characterization of a spliced exon product of herpes simplex type-1 latency-associated transcript in productively infected cells. Publishing Authors By Initials

W Kang,R Mukerjee,JJ Gartner,AG Hatzigeorgiou,RM Sandri-Goldin,NW Fraser,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus latency research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus latency research

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Characterization of a spliced exon product of herpes simplex type-1 latency-associated transcript in productively infected cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Virology

VOLUME: 356

Page Numbers: 106-14

Journal Abbreviation: Virology

ISSN: 0042-6822

DAY: 30

MONTH: 08

YEAR: 2006

Characterization of a spliced exon product of herpes simplex type-1 latency-associated transcript in productively infected cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 110674

Characterization of a spliced exon product of herpes simplex type-1 latency-associated transcript in productively infected cells. Keywords Mesh Terms:

KEYWORDS: Virus Latency

MESH TERMS: metabolism

Chemical & Substance for Abstract: Characterization of a spliced exon product of herpes simplex type-1 latency-associated transcript in productively infected cells. Information

Substance Name: latency associated transcript protein, h

Registry Number: 0

Grant and Affiliation Information for Characterization of a spliced exon product of herpes simplex type-1 latency-associated transcript in productively infected cells.

AFFILIATION: Department of Microbiology, University of Pennsylvania Medical School, 315 Johnson Pavilion, Philadelphia, PA 19104, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS 33768

ACRONYM: NS

MEDLINETA: Virology

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