Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses.

Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses. Research Abstract Details 

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Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses. Abstract Text:

Susan Parrish,Bernard Moss,Susan Parrish,Bernard Moss,

Vaccinia virus (VACV) encodes enzymes that cap the 5' end of viral mRNAs, which enhances their stability and translation. Nevertheless, recent studies demonstrated that the VACV D10 protein (VACV-WR_115) decaps mRNA, an enzymatic activity not previously shown to be encoded by a virus. The decapping activity of D10 is dependent on a Nudix hydrolase motif that is also present in the VACV D9 protein (VACV-WR_114), which shares 25% sequence identity with D10. Here, we showed that a purified recombinant VACV D9 fusion protein also decaps mRNA and that this activity was abolished by point mutations in the Nudix hydrolase motif. Decapping was specific for a methylated cap attached to RNA and resulted in the liberation of m7GDP. D9 differed from D10 in requiring a longer capped RNA substrate for optimal activity, having greater sensitivity to inhibition by uncapped RNA, and having lower sensitivity to inhibition by nucleotide cap analogs unattached to RNA. Since D9 is expressed early in infection and D10 late, we suggest that the two proteins enhance mRNA turnover and manipulate gene expression in a complementary and overlapping manner.

Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses. Publishing Authors By Initials

S Parrish,B Moss,S Parrish,B Moss,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Intr

Journal: Journal of virology

VOLUME: 81

Page Numbers: 12973-8

Journal Abbreviation: J. Virol.

ISSN: 1098-5514

DAY: 19

MONTH: 09

YEAR: 2007

Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses. Information

Substance Name: Cap(m(7)GpppXm) endonuclease

Registry Number: EC 3.1.27.-

Grant and Affiliation Information for Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses.

AFFILIATION: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 33 North Drive, Bethesda, MD 20892-3210, USA.

Country: United States

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MEDLINETA: J Virol

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