Characterization of a Novel Prokaryotic GDP Dissociation Inhibitor Domain from the G Protein Coupled Membrane Protein FeoB.

Characterization of a Novel Prokaryotic GDP Dissociation Inhibitor Domain from the G Protein Coupled Membrane Protein FeoB. Research Abstract Details 

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Characterization of a Novel Prokaryotic GDP Dissociation Inhibitor Domain from the G Protein Coupled Membrane Protein FeoB. Abstract Text:

Edward T Eng,Amir R Jalilian,Krasimir A Spasov,Vinzenz M Unger,Edward T Eng,Amir R Jalilian,Krasimir A Spasov,Vinzenz M Unger,Edward T Eng,Amir R Jalilian,Krasimir A Spasov,Vinzenz M Unger,

The FeoB family of membrane embedded G proteins are involved with high affinity Fe(II) uptake in prokaryotes. Here, we report that FeoB harbors a novel GDP dissociation inhibitor-like domain that specifically stabilizes GDP-binding through an interaction with the switch I region of the G protein. We show that the stabilization of GDP binding is conserved between species despite a high degree of sequence variability in their guanine nucleotide dissociation inhibitor (GDI)-like domains, and demonstrate that the presence of the membrane embedded domain increases GDP-binding affinity roughly 150-fold over the level accomplished by action of the GDI-like domain alone. To our knowledge, this is the first example for a prokaryotic GDI, targeting a bacterial G protein-coupled membrane process. Our findings suggest that Fe(II) uptake in bacteria involves a G protein regulatory pathway reminiscent of signaling mechanisms found in higher-order organisms.

Characterization of a Novel Prokaryotic GDP Dissociation Inhibitor Domain from the G Protein Coupled Membrane Protein FeoB. Publishing Authors By Initials

ET Eng,AR Jalilian,KA Spasov,VM Unger,ET Eng,AR Jalilian,KA Spasov,VM Unger,ET Eng,AR Jalilian,KA Spasov,VM Unger,

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Characterization of a Novel Prokaryotic GDP Dissociation Inhibitor Domain from the G Protein Coupled Membrane Protein FeoB. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of molecular biology

VOLUME: 375

Page Numbers: 1086-97

Journal Abbreviation: J. Mol. Biol.

ISSN: 1089-8638

DAY: 19

MONTH: 11

YEAR: 2007

Characterization of a Novel Prokaryotic GDP Dissociation Inhibitor Domain from the G Protein Coupled Membrane Protein FeoB. Information

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LANGUAGE: eng

NlmUniqueID: 2985088

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Grant and Affiliation Information for Characterization of a Novel Prokaryotic GDP Dissociation Inhibitor Domain from the G Protein Coupled Membrane Protein FeoB.

AFFILIATION: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, PO Box 208024, New Haven, CT 06520-8024, USA.

Country: England

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MEDLINETA: J Mol Biol

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