Characterization of a novel gene for strain typing reveals substructuring of Aspergillus fumigatus across North America.

Characterization of a novel gene for strain typing reveals substructuring of Aspergillus fumigatus across North America. Research Abstract Details 

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Characterization of a novel gene for strain typing reveals substructuring of Aspergillus fumigatus across North America. Abstract Text:

S Arunmozhi Balajee,Sun T Tay,Brent A Lasker,Steve F Hurst,Alejandro P Rooney,

Fifty-five epidemiologically linked Aspergillus fumigatus isolates obtained from six nosocomial outbreaks of invasive aspergillosis were subtyped by sequencing the polymorphic region of the gene encoding a putative cell surface protein, Afu3g08990 (denoted as CSP). Comparative sequence analysis showed that genetic diversity was generated in the coding region of this gene by both tandem repeats and point mutations. Each unique sequence in an outbreak cluster was assigned an arbitrary number or CSP sequence type. The CSP typing method was able to identify "clonal" and genotypically distinct A. fumigatus isolates, and the results of this method were concordant with those of another discriminatory genotyping technique, the Afut1 restriction fragment length polymorphism typing method. The novel single-locus sequence typing (CSP typing) strategy appears to be a simple, rapid, discriminatory tool that can be readily shared across laboratories. In addition, we found that A. fumigatus isolates substructured into multiple clades; interestingly, one clade consisted of isolates predominantly representing invasive clinical isolates recovered from cardiac transplant patients from two different outbreak situations. We also found that the A. fumigatus isolate Af293, whose genome has been sequenced, possesses a CSP gene structure that is substantially different from those of the other A. fumigatus strains studied here, highlighting the need for further taxonomic study.

Characterization of a novel gene for strain typing reveals substructuring of Aspergillus fumigatus across North America. Publishing Authors By Initials

SA Balajee,ST Tay,BA Lasker,SF Hurst,AP Rooney,

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Characterization of a novel gene for strain typing reveals substructuring of Aspergillus fumigatus across North America. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Eukaryotic cell

VOLUME: 6

Page Numbers: 1392-9

Journal Abbreviation: Eukaryotic Cell

ISSN: 1535-9778

DAY: 8

MONTH: 06

YEAR: 2007

Characterization of a novel gene for strain typing reveals substructuring of Aspergillus fumigatus across North America. Information

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LANGUAGE: eng

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AFFILIATION: Mycotic Diseases Branch, Centers for Disease Control and Prevention, Mail stop G 11, 1600 Clifton Road, Atlanta, GA 30333, USA. fir3@cdc.gov

Country: United States

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MEDLINETA: Eukaryot Cell

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