Characterization of a novel fungal protein, p15, which induces neuronal differentiation of PC12 cells.

Characterization of a novel fungal protein, p15, which induces neuronal differentiation of PC12 cells. Research Abstract Details 

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Characterization of a novel fungal protein, p15, which induces neuronal differentiation of PC12 cells. Abstract Text:

S Wakatsuki,M Arioka,N Dohmae,K Takio,M Yamasaki,K Kitamoto,

In our previous paper, we reported that a 15 kDa protein (p15) produced by a fungus, genus Helicosporium, enhanced NGF-induced neurite outgrowth from PC12 cells. Here we further characterized the actions of p15. The complete amino acid sequence of p15 was determined and it was shown to be a hydrophilic protein composed of 118 amino acid residues with two intramolecular disulfide bridges. p15-induced neurite outgrowth was blocked by the depletion of extracellular Ca(2+) in the culture medium and was significantly inhibited by L-type Ca(2+) channel inhibitor nicardipine. p15 stimulated Src kinase and MAPK activities, and neurite outgrowth was not observed in srcDN2, a dominant negative c-src(K295R)-expressing cell line, and was significantly reduced in RasN17-expressing cells. These results suggest that p15 stimulates neurite outgrowth through the potentiation of L-type Ca(2+) channels, thereby activating the Src-Ras-MAPK cascade.

Characterization of a novel fungal protein, p15, which induces neuronal differentiation of PC12 cells. Publishing Authors By Initials

S Wakatsuki,M Arioka,N Dohmae,K Takio,M Yamasaki,K Kitamoto,

For similar proteins: neoplasm proteins: tumor suppressor proteins research abstracts see: proteins: neoplasm proteins: tumor suppressor proteins research

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Characterization of a novel fungal protein, p15, which induces neuronal differentiation of PC12 cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 126

Page Numbers: 1151-60

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1999

Characterization of a novel fungal protein, p15, which induces neuronal differentiation of PC12 cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Characterization of a novel fungal protein, p15, which induces neuronal differentiation of PC12 cells. Keywords Mesh Terms:

KEYWORDS: Tumor Suppressor Proteins

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Characterization of a novel fungal protein, p15, which induces neuronal differentiation of PC12 cells. Information

Substance Name: Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Characterization of a novel fungal protein, p15, which induces neuronal differentiation of PC12 cells.

AFFILIATION: Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan.

Country: JAPAN

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MEDLINETA: J Biochem

REFSOURCE: J Biochem (Tokyo) 2000 May;127(5):939

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