Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster.

Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster. Research Abstract Details 

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Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster. Abstract Text:

Zachary D Aron,Pieter C Dorrestein,Jonathan R Blackhall,Neil L Kelleher,Christopher T Walsh,

We report the expression and characterization of a truncated form of MycA from the Mycosubtilin gene cluster from Bacillus subtilis. The MycA fragment contains a new amino transferase (AMT) tailoring domain, allowing the first detailed study of a PLP-dependent enzyme operating in cis within the PKS and NRPS biosynthetic paradigm. As the AMT domain acts on covalently bound beta-ketothioesters, and is therefore a single-turnover system, electrospray ionization-Fourier transform mass spectrometry (ESI-FTMS) was used to observe the amine-transfer reaction both for amine donor substrate specificity and to regiospecifically determine enzyme-bound intermediates. We confirm the function of the AMT domain, dissect the mechanistic steps of amine transfer, identify the preferred amine source, and localize the beta-ketothioester substrate during amine transfer.

Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster. Publishing Authors By Initials

ZD Aron,PC Dorrestein,JR Blackhall,NL Kelleher,CT Walsh,

For similar enzymes and coenzymes: enzymes: transferases: nitrogenous group transferases: transaminases research abstracts see: enzymes and coenzymes: enzymes: transferases: nitrogenous group transferases: transaminases research

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Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 14986-7

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 2

MONTH: Nov

YEAR: 2005

Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster. Keywords Mesh Terms:

KEYWORDS: Transaminases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster. Information

Substance Name: Transaminases

Registry Number: EC 2.6.1.-

Grant and Affiliation Information for Characterization of a new tailoring domain in polyketide biogenesis: the amine transferase domain of MycA in the mycosubtilin gene cluster.

AFFILIATION: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM067725

ACRONYM: GM

MEDLINETA: J Am Chem Soc

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