Characterization of a membrane-bound arginine-specific serine protease from rat intestinal mucosa.

Characterization of a membrane-bound arginine-specific serine protease from rat intestinal mucosa. Research Abstract Details 

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Characterization of a membrane-bound arginine-specific serine protease from rat intestinal mucosa. Abstract Text:

K Kishi,K Yamazaki,I Yasuda,N Yahagi,M Ichinose,Y Tsuchiya,S B Athauda,H Inoue,K Takahashi,

Previously we isolated and characterized a membrane-bound, arginine-specific serine protease from pig intestinal mucosa [J. Biol. Chem. 269, 32985-32991 (1994)]. For further characterization of this type of enzyme, we cloned a cDNA from rat intestinal mucosa encoding the precursor of a similar protease. The partial amino acid sequences determined for the pig enzyme were found to be shared almost completely by the rat enzyme. The serine protease domain of the rat enzyme, heterologously expressed in Escherichia coli, specifically cleaved Arg (or Lys)-X bonds with a marked preference for Arg-Arg or Arg-Lys, similar to the pig enzyme. The mRNA for the rat enzyme was shown to be distributed mainly in intestine, and the enzyme was detected in the duodenal mucosa as a 70 kDa protein. Immunohistochemical analysis of the small intestinal tissue showed that the enzyme is localized mainly on brushborder membranes.

Characterization of a membrane-bound arginine-specific serine protease from rat intestinal mucosa. Publishing Authors By Initials

K Kishi,K Yamazaki,I Yasuda,N Yahagi,M Ichinose,Y Tsuchiya,SB Athauda,H Inoue,K Takahashi,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: trypsin research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: trypsin research

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Characterization of a membrane-bound arginine-specific serine protease from rat intestinal mucosa. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 130

Page Numbers: 425-30

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 2001

Characterization of a membrane-bound arginine-specific serine protease from rat intestinal mucosa. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Characterization of a membrane-bound arginine-specific serine protease from rat intestinal mucosa. Keywords Mesh Terms:

KEYWORDS: Trypsin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Characterization of a membrane-bound arginine-specific serine protease from rat intestinal mucosa. Information

Substance Name: Trypsin

Registry Number: EC 3.4.21.4

Grant and Affiliation Information for Characterization of a membrane-bound arginine-specific serine protease from rat intestinal mucosa.

AFFILIATION: School of Life Science, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo 192-0392, Japan.

Country: Japan

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MEDLINETA: J Biochem

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ACCESSION NUMBER: AB049189

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