Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen.

Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen. Abstract Text:

S Wagner,J Freudenstein,K H Scheit,

A cDNA library derived from poly(A)+RNA of bull seminal vesicle tissue was screened with synthetic DNA probes specific for seminalplasmin (SAP), the major basic protein of bull semen. From a number of positive clones, pBSV12, containing a 577-bp insert, was identified and sequenced. The derived amino acid sequence comprises the known amino acid sequence of SAP with an amino terminal representing a putative signal sequence; at the carboxyl terminus the sequence contains an additional lysine residue. Present experimental data do not distinguish between two potential SAP precursor molecules, each starting with a methionine residue and differing by 10 amino acid residues in the leader peptide. Comparative Northern analysis reveals a SAP-specific mRNA of 700 bp, which lacks RNA from bovine testis as well as from seminal vesicle tissue of a bull calf; hence, expression of the SAP gene appears to be under androgen and/or developmental control. Southern analysis indicates that one gene appears to specify SAP. SAP-like DNA sequences were detected in ovine and porcine genomic DNA.

Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen. Publishing Authors By Initials

S Wagner,J Freudenstein,KH Scheit,

For similar proteins: seminal plasma proteins: seminal vesicle secretory proteins research abstracts see: proteins: seminal plasma proteins: seminal vesicle secretory proteins research

PUBMED ID PMID:

MEDLINE DATE: 1990 Jul-Aug

Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: DNA and cell biology

VOLUME: 9

Page Numbers: 437-42

Journal Abbreviation: DNA Cell Biol.

ISSN: 1044-5498

DAY: 19

MONTH: 11

YEAR: 2007

Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9004522

Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen. Keywords Mesh Terms:

KEYWORDS: Seminal Vesicle Secretory Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen.

AFFILIATION: Max-Planck-Institut für biophysikalische Chemie, Göttingen, West Germany.

Country: UNITED STATES

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: DNA Cell Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen Related Publications

• Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen.
• Characterization of the gene for seminalplasmin, a secretory protein of the bovine seminal vesicle.
• Effect of seminal plasmin on rRNA synthesis in Saccharomyces cerevisiae.
• Functional properties of peptides derived from seminalplasmin: binding to monospecific anti-seminalplasmin immunoglobulins G and calmodulin.
• Immunohistochemistry of secretory proteins in the bull seminal vesicle.
• Immunological identification of seminalplasmin in tissue extracts of sex glands of bull.
• Promoter analysis of the bovine gene for seminalplasmin.
• Purification and spectral characterization of seminalplasmin, an antimicrobial protein from bull semen.
• Seminalplasmin, the major basic protein of bull seminal plasma, is a secretory protein of the seminal vesicles.
• Synthesis of bioactive seminalplasmin by expression of a newly constructed fusion gene.
• [A quantitative ELISA for the measurement of class-specific anti-spermatozoa antibodies]