Characterization and in vitro cytotoxicity testing of ethanolamine-derived cadmium chelating agents.

Characterization and in vitro cytotoxicity testing of ethanolamine-derived cadmium chelating agents. Research Abstract Details 

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Characterization and in vitro cytotoxicity testing of ethanolamine-derived cadmium chelating agents. Abstract Text:

Mikhail Zhukalin,Megan K Blanksma,T Dimitri Silva,Samuel W Suyehira,Wendy A Harvey,Sara J Heggland,Peter R Craig,

We have synthesized and characterized the new cadmium chelating agent potassium bis(2-hydroxyethyl)aminoethyldithiocarbonate hemihydrate, K[bhexan] x 0.5H2O (2), that is structurally related to the known effective in vivo cadmium chelating agent potassium bis(2-hydroxyethyl)dithiocarbamate, K[bhedtc] (1). The corresponding cadmium complex of 2 differs from di(bis(2-hydroxyethyl)dithiocarbamato)cadmium(II), Cd(bhedtc)2 (3), in that the insoluble compound exhibits an elemental composition consistent with a cadmium:ligand ratio of 2:1. The cytotoxicity of the 1-3 was investigated using the human osteoblast-like cell line, Saos-2. Compounds 1 or 2 did not affect cell adherence or cell viability in the 100-500 microM concentration range studied, whereas 3 resulted in a concentration-dependent increase in loss of cell adherence and decrease in cell viability. Overall, the results of the loss of cell adherence, trypan blue exclusion and MTT assays showed that administration of 3 (cadmium complex of 1) resulted in cytotoxicity lower than that of cadmium chloride, but higher than that of the chelator 1 alone. The effect of simultaneous addition of cadmium chloride and 1 or 2 on cell viability was also assessed using the MTT assay. For the 100 microM cadmium chloride experiments, cell viability comparable to control cells was achieved for both 1 and 2 in the 100-500 microM concentration range studied. Cell viability comparable to control cells was achieved for 1 but not 2 in the 100-500 microM concentration range studied for the 200 microM cadmium chloride experiments. Thus 1 appears more effective than 2 in the ability to mediate the cytotoxic effects of cadmium in vitro upon concomitant administration.

Characterization and in vitro cytotoxicity testing of ethanolamine-derived cadmium chelating agents. Publishing Authors By Initials

M Zhukalin,MK Blanksma,TD Silva,SW Suyehira,WA Harvey,SJ Heggland,PR Craig,

For similar organic chemicals: carboxylic acids: acids, acyclic: carbamates: thiocarbamates research abstracts see: organic chemicals: carboxylic acids: acids, acyclic: carbamates: thiocarbamates research

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Characterization and in vitro cytotoxicity testing of ethanolamine-derived cadmium chelating agents. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biometals : an international journal on the role o

VOLUME: 20

Page Numbers: 61-72

Journal Abbreviation: Biometals

ISSN: 0966-0844

DAY: 7

MONTH: 06

YEAR: 2006

Characterization and in vitro cytotoxicity testing of ethanolamine-derived cadmium chelating agents. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9208478

Characterization and in vitro cytotoxicity testing of ethanolamine-derived cadmium chelating agents. Keywords Mesh Terms:

KEYWORDS: Thiocarbamates

MESH TERMS: chemistry

Chemical & Substance for Abstract: Characterization and in vitro cytotoxicity testing of ethanolamine-derived cadmium chelating agents. Information

Substance Name: Cadmium

Registry Number: 7440-43-9

Grant and Affiliation Information for Characterization and in vitro cytotoxicity testing of ethanolamine-derived cadmium chelating agents.

AFFILIATION: Departments of Chemistry, Albertson College of Idaho, Caldwell, Idaho 83605-4432, USA.

Country: Netherlands

AGENCY: United States NCRR

GRANT: P20 RR016454

ACRONYM: RR

MEDLINETA: Biometals

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