Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus.

Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus. Research Abstract Details 

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Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus. Abstract Text:

Hiroaki Kariwa,Hiroshi Noda,Mina Nakauchi,Mariko Ishizuka,Kazuaki Hashiguchi,Shingo Hashimoto,Kentaro Yoshii,Atsushi Asano,Takashi Agui,Hiroyuki Kogaki,Yoshihiro Kurano,Yoshiaki Uchida,Nobuyuki Fujii,Masahisa Okada,Ikuo Takashima,

The sudden emergence of severe acute respiratory syndrome (SARS) at the end of 2002 resulted in 774 reported deaths from more than 8000 cases worldwide. As no effective vaccines or antiviral agents are available, the most effective measure to prevent the expansion of a SARS epidemic is the rapid diagnosis and isolation of SARS patients. To establish specific diagnostic methods, we generated nine clones of monoclonal antibodies to nucleocapsid protein (NP) of SARS-coronavirus (SARS-CoV). On immunofluorescent antibody assay and Western blotting analysis, none of the monoclonal antibodies showed cross-reactivity to authentic and recombinant NPs of human coronavirus (HCoV) 229E strain. To determine the region on the NP molecule where the monoclonal antibodies bind, we generated four truncated recombinant NPs and analyzed the reactivity between monoclonal antibodies and truncated NPs. Two monoclonal antibodies reacted with a truncated NP covering from amino acid residues 111 to 230, and seven reacted with another truncated NP covering from amino acid residues 221 to 340. Epitope mapping analysis indicated that monoclonal antibody SN5-25 recognized the amino acid sequence Q(245)TVTKK(250) On SARS-NP. Within the epitope, Q245, T246, V247, K249, and K250 appeared to form an essential motif for monoclonal antibody SN5-25 to bind. The information about binding sites and epitopes of monoclonal antibodies may be useful for the development of new diagnostic methods for SARS and for analyzing the function of N protein of SARS-CoV.

Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus. Publishing Authors By Initials

H Kariwa,H Noda,M Nakauchi,M Ishizuka,K Hashiguchi,S Hashimoto,K Yoshii,A Asano,T Agui,H Kogaki,Y Kurano,Y Uchida,N Fujii,M Okada,I Takashima,

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Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Japanese journal of veterinary research

VOLUME: 55

Page Numbers: 115-27

Journal Abbreviation: Jpn. J. Vet. Res.

ISSN: 0047-1917

DAY: 2

MONTH: Feb

YEAR: 2008

Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus. Information

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LANGUAGE: eng

NlmUniqueID: 376567

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Grant and Affiliation Information for Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirus.

AFFILIATION: Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. kariwa@vetemed.hokudai.ac.jp

Country: Japan

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MEDLINETA: Jpn J Vet Res

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