Characterization and application of a novel RNA aptamer against the mouse prion protein.

Characterization and application of a novel RNA aptamer against the mouse prion protein. Research Abstract Details 

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Characterization and application of a novel RNA aptamer against the mouse prion protein. Abstract Text:

Satoru Sekiya,Ken Noda,Fumiko Nishikawa,Takashi Yokoyama,Penmetcha K R Kumar,Satoshi Nishikawa,

In order to isolate RNA aptamers against the mouse prion protein (mPrP), we carried out in vitro selection from RNA pools containing a 30-nucleotide randomized region. Aptamer 60-3 was found to have a high affinity for mPrP (K(d) = 5.6 +/- 1.5 nM), and 2'-fluoro-pyrimidine modifications for RNase resistance did not abolish its binding activity (K(d) = 22 +/- 4 nM). Following 5' biotinylation, aptamer 60-3 specifically detected PrP in mouse brain homogenate in a Northwestern blotting assay. To determine the mPrP-aptamer binding region, we performed protein-deletion-mutant analysis and competition-binding analysis using heparin. The results showed that aptamer 60-3 appears to have binding sites located between amino acids 23-108.

Characterization and application of a novel RNA aptamer against the mouse prion protein. Publishing Authors By Initials

S Sekiya,K Noda,F Nishikawa,T Yokoyama,PK Kumar,S Nishikawa,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research

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Characterization and application of a novel RNA aptamer against the mouse prion protein. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 139

Page Numbers: 383-90

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 2006

Characterization and application of a novel RNA aptamer against the mouse prion protein. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Characterization and application of a novel RNA aptamer against the mouse prion protein. Keywords Mesh Terms:

KEYWORDS: RNA

MESH TERMS: metabolism

Chemical & Substance for Abstract: Characterization and application of a novel RNA aptamer against the mouse prion protein. Information

Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for Characterization and application of a novel RNA aptamer against the mouse prion protein.

AFFILIATION: Cooperative Graduate School, University of Tsukuba, Tennodai, Tsukuba, Ibaraki.

Country: Japan

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MEDLINETA: J Biochem

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