Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes.

Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes. Research Abstract Details 

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Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes. Abstract Text:

Hangxiu Xu,Drina D Sta Iglesia,Jennifer L Kielczewski,Danielle F Valenta,Mary E Pease,Donald J Zack,Harry A Quigley,

PURPOSE: To isolate and characterize progenitor cells derived from adult mammalian ciliary body. METHODS: The authors isolated progenitor cells from the ciliary body of adult mice, rats, and human cadaver eyes and determined quantitative growth characteristics of groups of progenitor cells called neurosphere (NS) cells, including individual cell diameter, NS diameter, percentage of NS-forming cells, and cell number per eye in mouse, rat, and human eyes. The immunolabeling and ultrastructure of NS cells were investigated by confocal and transmission electron microscopy. RESULTS: Average diameters of individual cells and neurospheres after 1 week in culture were similar in mice, rats, and humans (cell diameters: 22 +/- 1.1, 21 +/- 0.3, 25 +/- 0.4 mum; NS diameters: 139 +/- 22, 137 +/- 9, 141 +/- 11 mum, respectively). Mean numbers of cells per NS were estimated to be 1183 in mice, 5360 in rats, and 685 in humans. Molecules that were identified by immunolabeling in NS cells included nestin, Chx-10, vimentin, GFAP, and Pax-6. Thy-1 was expressed in some NS cells. Ultrastructurally, NS cells displayed abundant rough endoplasmic reticulum and many cellular processes but no characteristics of mature retinal neurons or glia. CONCLUSIONS: Progenitor cells from adult mammalian ciliary body have significant, but limited, proliferation potential and express markers characteristic of other progenitor cells and seen during early retinal development. The ciliary body could be a source of cells for transplantation in experimental rodent eyes and for autotransplantation in human eyes.

Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes. Publishing Authors By Initials

H Xu,DD Sta Iglesia,JL Kielczewski,DF Valenta,ME Pease,DJ Zack,HA Quigley,

For similar macromolecular substances: polymers: biopolymers: intermediate filament proteins: vimentin research abstracts see: macromolecular substances: polymers: biopolymers: intermediate filament proteins: vimentin research

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Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Investigative ophthalmology & visual science

VOLUME: 48

Page Numbers: 1674-82

Journal Abbreviation: Invest. Ophthalmol. Vis. Sci.

ISSN: 0146-0404

DAY: 3

MONTH: Apr

YEAR: 2007

Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7703701

Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes. Keywords Mesh Terms:

KEYWORDS: Vimentin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes. Information

Substance Name: nestin

Registry Number: 0

Grant and Affiliation Information for Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes.

AFFILIATION: Glaucoma Research Laboratory, Wilmer Eye Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA. hxu13@jhmi.edu

Country: United States

AGENCY: United States NEI

GRANT: P30 EY 001765

ACRONYM: EY

MEDLINETA: Invest Ophthalmol Vis Sci

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