Characteristics of detergent-resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene.

Characteristics of detergent-resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene. Research Abstract Details 

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Characteristics of detergent-resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene. Abstract Text:

H Homma,N Chiba,T Kobayashi,I Kudo,K Inoue,H Ikeda,M Sekiguchi,S Nojima,

Detergent-resistant phospholipase A (DR-phospholipase A) of E. coli is a 28K-dalton protein and is exclusively located in the outer membrane. We cloned the pldA gene of E. coli, which is responsible for the activity of DR-phospholipase A. Strains bearing the plasmid which contained the pldA gene yielded a large amount of the outer membrane protein with a molecular weight of about 28K daltons and overproduced 20 to 65 times as much DR-phospholipase A activity as the wild type strain. Experiments with minicells and maxicells revealed that the pldA-containing plasmid was coding for a 28K protein. These results strongly indicated that pldA is the structural gene for DR-phospholipase A. There was apparently no difference with respect to the association of the enzyme with the envelope fraction between the overproducer and the wild type strain. The overproduced enzyme was properly transported to the outer membrane. Neither the growth rate nor the phospholipid composition of the overproducer was remarkably different from in the wild type strain. Thus, the overproduction of DR-phospholipase A apparently caused no phenotypic variations. E. coli has very excessive ability to transport and integrate the outer membrane protein.

Characteristics of detergent-resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene. Publishing Authors By Initials

H Homma,N Chiba,T Kobayashi,I Kudo,K Inoue,H Ikeda,M Sekiguchi,S Nojima,

For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

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Characteristics of detergent-resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 96

Page Numbers: 1645-53

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1984

Characteristics of detergent-resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Characteristics of detergent-resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene. Keywords Mesh Terms:

KEYWORDS: Plasmids

MESH TERMS: analysis

Chemical & Substance for Abstract: Characteristics of detergent-resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene. Information

Substance Name: Phospholipases A

Registry Number: EC 3.1.1.-

Grant and Affiliation Information for Characteristics of detergent-resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene.

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Country: JAPAN

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MEDLINETA: J Biochem

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