Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporter.

Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporter. Research Abstract Details 

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Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporter. Abstract Text:

Fiona Rae,Kyra Woods,Tedjo Sasmono,Naomi Campanale,Darrin Taylor,Dmitry A Ovchinnikov,Sean M Grimmond,David A Hume,Sharon D Ricardo,Melissa H Little,

All solid organs contain resident monocyte-derived cells that appear early in organogenesis and persist throughout life. These cells are critical for normal development in some organs. Here we report the use of a previously described transgenic line, with EGFP driven by the macrophage-restricted Csf1r (c-fms) promoter, to image macrophage production and infiltration accompanying organogenesis in many tissues. Using microarray analysis of FACS-isolated EGFP-positive cells, we show that fetal kidney, lung and brain macrophages show similar gene expression profiles irrespective of their tissue of origin. EGFP-positive cells appeared in the renal interstitium from 12 days post coitum, prior to nephrogenesis, and maintain a close apposition to renal tubules postnatally. CSF-1 added to embryonic kidney explants increased overall renal growth and ureteric bud branching. Expression profiling of tissue macrophages and of CSF-1-treated explants showed evidence of the alternate, pro-proliferative (M2) activation profile, including expression of macrophage mannose receptor (CD206), macrophage scavenger receptor 2 (Msr2), C1q, CD163, selenoprotein P, CCL24 and TREM2. This response has been associated with the trophic role of tumour-associated macrophages. These findings suggest a trophic role of macrophages in embryonic kidney development, which may continue to play a similar role in postnatal repair.

Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporter. Publishing Authors By Initials

F Rae,K Woods,T Sasmono,N Campanale,D Taylor,DA Ovchinnikov,SM Grimmond,DA Hume,SD Ricardo,MH Little,

For similar proteins: recombinant proteins: recombinant fusion proteins research abstracts see: proteins: recombinant proteins: recombinant fusion proteins research

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Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporter. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Developmental biology

VOLUME: 308

Page Numbers: 232-46

Journal Abbreviation: Dev. Biol.

ISSN: 0012-1606

DAY: 25

MONTH: 05

YEAR: 2007

Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporter. Information

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LANGUAGE: eng

NlmUniqueID: 372762

Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporter. Keywords Mesh Terms:

KEYWORDS: Recombinant Fusion Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporter. Information

Substance Name: Receptor, Macrophage Colony-Stimulating

Registry Number: EC 2.7.1.112

Grant and Affiliation Information for Characterisation and trophic functions of murine embryonic macrophages based upon the use of a Csf1r-EGFP transgene reporter.

AFFILIATION: Institute for Molecular Bioscience and ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Queensland 4072, Australia.

Country: United States

AGENCY: United States NIDDK

GRANT: DK63400

ACRONYM: DK

MEDLINETA: Dev Biol

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