Changing pattern of the subcellular distribution of erythroblast macrophage protein (Emp) during macrophage differentiation.

Changing pattern of the subcellular distribution of erythroblast macrophage protein (Emp) during macrophage differentiation. Research Abstract Details 

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Changing pattern of the subcellular distribution of erythroblast macrophage protein (Emp) during macrophage differentiation. Abstract Text:

Shivani Soni,Shashi Bala,Ajay Kumar,Manjit Hanspal,

Erythroblast macrophage protein (Emp) mediates the attachment of erythroid cells to macrophages and is required for normal differentiation of both cell lineages. In erythroid cells, Emp is believed to be involved in nuclear extrusion, however, its role in macrophage differentiation is unknown. Information on the changes in the expression level and subcellular distribution of Emp in differentiating macrophages is essential for understanding the function of Emp. Macrophages of varying maturity were examined by immunofluorescence microscopy and biochemical methods. Our data show that Emp is expressed in all stages of maturation, but its localization pattern changes dramatically during maturation: in immature macrophages, a substantial fraction of Emp is associated with the nuclear matrix, whereas in more mature cells, Emp is expressed largely at cell surface. Pulse-chase experiments show that nascent Emp migrates intracellularly from the cytoplasm to the plasma membrane more efficiently in mature macrophages than in immature cells. Incubation of erythroid cells with macrophages in culture shows that erythroid cells attach to mature macrophages but not to immature macrophage precursors. Together, our data show that the temporal and spatial expression of Emp correlates with its role in erythroblastic island formation and suggest that Emp may be involved in multiple cellular functions.

Changing pattern of the subcellular distribution of erythroblast macrophage protein (Emp) during macrophage differentiation. Publishing Authors By Initials

S Soni,S Bala,A Kumar,M Hanspal,

For similar cells: cellular structures: subcellular fractions research abstracts see: cells: cellular structures: subcellular fractions research

PUBMED ID PMID:

MEDLINE DATE: 2007 Jan-Feb

Changing pattern of the subcellular distribution of erythroblast macrophage protein (Emp) during macrophage differentiation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Blood cells, molecules & diseases

VOLUME: 38

Page Numbers: 25-31

Journal Abbreviation: Blood Cells Mol. Dis.

ISSN: 1079-9796

DAY: 27

MONTH: 10

YEAR: 2006

Changing pattern of the subcellular distribution of erythroblast macrophage protein (Emp) during macrophage differentiation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9509932

Changing pattern of the subcellular distribution of erythroblast macrophage protein (Emp) during macrophage differentiation. Keywords Mesh Terms:

KEYWORDS: Subcellular Fractions

MESH TERMS: metabolism

Chemical & Substance for Abstract: Changing pattern of the subcellular distribution of erythroblast macrophage protein (Emp) during macrophage differentiation. Information

Substance Name: erythroblast macrophage protein, mouse

Registry Number: 0

Grant and Affiliation Information for Changing pattern of the subcellular distribution of erythroblast macrophage protein (Emp) during macrophage differentiation.

AFFILIATION: Department of Medicine, Center for Cell Biology, CBR 406, Caritas St. Elizabeth's Medical Center, Tufts University School of Medicine, Boston, MA 02135, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI050600-05

ACRONYM: AI

MEDLINETA: Blood Cells Mol Dis

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