[Changes of the migration ability of the cord blood CD(34)(+) cells during short-term ex vivo expansion]

[Changes of the migration ability of the cord blood CD(34)(+) cells during short-term ex vivo expansion] Research Abstract Details 

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[Changes of the migration ability of the cord blood CD(34)(+) cells during short-term ex vivo expansion] Abstract Text:

Qiong-Li Zhai,Ya-Fei Wang,Qian Li,Yong-Jun Liu,Zhen Yu,Yu Zhou,Heng-Xing Meng,Lu-Gui Qiu,Zhong-Chao Han,

OBJECTIVE: To study the effect of ex vivo expansion on the migration ability and the CXCR4 expression of umbilical cord blood (CB) hematopoietic stem/progenitor cells (HSPC). METHODS: CD(34)(+) cells isolated from fresh CB samples were cultured in a serum-free and stroma-free culture system. On day 7, 10 and 14, CD(34)(+) cells were re-selected from the expanded cells, and the expression of CXCR4 and the transmigration ability of these CD(34)(+) cells were evaluated respectively and compared with those of the precultured fresh CD(34)(+) cells. RESULTS: (1) SDF-1 induced a higher migration percentage of fresh or expanded CB CD(34)(+) cells than that of uninduced ones. (2) Both of the uninduced and SDF-1-induced migrations were slightly reduced in the first week and then much more reduced in the second week expansion (P < 0.05). (3) The number of the CD(34)(+)CXCR4(+) cells were significantly increased during the culture period, but there was a downtrend of CXCR4 expression on CD(34)(+) subset; the expression levels on day 10 and 14 were lower than that on day 0. CONCLUSIONS: The expanded HSPC would sustain the chemotactic activity during one-week-culture, but with further extended culture time their intrinsic homing potential would be partly impaired.

[Changes of the migration ability of the cord blood CD(34)(+) cells during short-term ex vivo expansion] Publishing Authors By Initials

QL Zhai,YF Wang,Q Li,YJ Liu,Z Yu,Y Zhou,HX Meng,LG Qiu,ZC Han,

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[Changes of the migration ability of the cord blood CD(34)(+) cells during short-term ex vivo expansion] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zaz

VOLUME: 25

Page Numbers: 163-6

Journal Abbreviation: Zhonghua Xue Ye Xue Za Zhi

ISSN: 0253-2727

DAY: 8

MONTH: Mar

YEAR: 2004

[Changes of the migration ability of the cord blood CD(34)(+) cells during short-term ex vivo expansion] Information

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LANGUAGE: chi

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AFFILIATION: State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020, China.

Country: China

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MEDLINETA: Zhonghua Xue Ye Xue Za Zhi

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