[Changes of sarco(endo)plasmic reticulum Ca2+-Mg2+-ATPase activity of cultured myoblast induced by cyclic stretch]

[Changes of sarco(endo)plasmic reticulum Ca2+-Mg2+-ATPase activity of cultured myoblast induced by cyclic stretch] Research Abstract Details 

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[Changes of sarco(endo)plasmic reticulum Ca2+-Mg2+-ATPase activity of cultured myoblast induced by cyclic stretch] Abstract Text:

Xiao-xia Che,Hong Zeng,Song-jiao Luo,

OBJECTIVE: To evaluate the effect of stretch on sarco(endo)plasmic reticulum Ca2+-Mg2+ ATPases activity and mRNA level and study the remodeling reaction of muscle in a variety of mechanical environments. METHODS: Myoblast from maxillofacial skeletal muscle of one-week-old male Sprague-Dawley rat was cultured and stretched cyclicly using a four-point bend device. Inorganic Phosphorus test was used to compare the activity of Ca2+-Mg2+ ATPases of myoblast before and after stretch. RT-PCR was also used to observe the Ca2+-Mg2+ ATPases mRNA level. RESULTS: The activity of Ca2+-Mg2+ ATPases of myoblast down regulated significantly in 4 hours. During the period of 8 hours to 24 hours, up-regulation followed then returned to control level at the 48 hour point. RT-PCR showed that Ca2+-Mg2+ ATPases mRNA level were elevated by stretch, particularly at 2 hour and 48 hour point. CONCLUSION: The results suggested a transcriptional control of Ca2+-Mg2+ ATPases activity was involved in the muscle remodeling process induced by stretch.

[Changes of sarco(endo)plasmic reticulum Ca2+-Mg2+-ATPase activity of cultured myoblast induced by cyclic stretch] Publishing Authors By Initials

XX Che,H Zeng,SJ Luo,

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[Changes of sarco(endo)plasmic reticulum Ca2+-Mg2+-ATPase activity of cultured myoblast induced by cyclic stretch] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yi

VOLUME: 22

Page Numbers: 281-3, 286

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ISSN: 1000-1182

DAY: 21

MONTH: Aug

YEAR: 2004

[Changes of sarco(endo)plasmic reticulum Ca2+-Mg2+-ATPase activity of cultured myoblast induced by cyclic stretch] Information

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LANGUAGE: chi

NlmUniqueID: 9422648

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Grant and Affiliation Information for [Changes of sarco(endo)plasmic reticulum Ca2+-Mg2+-ATPase activity of cultured myoblast induced by cyclic stretch]

AFFILIATION: Dept. of Orthodontics, Beijing Stomatology Hospital, Beijing 100050, China.

Country: China

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MEDLINETA: Hua Xi Kou Qiang Yi Xue Za Zhi

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