[Changes of phosphorylation states of Cx43 protein in Chinese hamster lung cells induced by SiO2]

[Changes of phosphorylation states of Cx43 protein in Chinese hamster lung cells induced by SiO2] Research Abstract Details 

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[Changes of phosphorylation states of Cx43 protein in Chinese hamster lung cells induced by SiO2] Abstract Text:

Weiwei Wu,Guogen Mao,Chen Yu,

OBJECTIVE: To investigate whether cellular gap-junctional communication(GJIC) down-regulation and the internalization of connexin 43(Cx43) in Chinese hamster lung fibroblasts (CHL) induced by silica-stimulated pulmonary alveolar macrophages (PAM) supermatant is related with the phosphorylation states of Cx43 protein. METHODS: Western-blot analysis was used to identify phosphorylated Cx43 species. RESULTS: Samples from membrane protein, total protein and nucleoprotein in CHL cells with 50-500 micrograms/ml doses of silica-stimulated PAM supernatants showed NP, P1, P2, P3 four immunoreactive bands of Cx43 protein by contrast with the control group and 0 microgram/ml SiO2 group. And with the dose of SiO2 increased, the increment of the levels of P2 and P3 was observed. Moreover, the groups treated with SiO2 and protein kinase C inhibitor, Palmitoyl-DL-Camitine chloride (PMC), simutaneously showed reduced level of P2 and P3, as compared with the groups treated with SiO2 only. CONCLUSION: The inhibition of GJIC and the internalization of Cx43 by SiO2 in CHL cells may relate to the changes of phosphorylation states of Cx43, and its mechanism may be similar to that of phorbol 12-myristate 13-acetate (TPA), i.e. via PKC activation pathway.

[Changes of phosphorylation states of Cx43 protein in Chinese hamster lung cells induced by SiO2] Publishing Authors By Initials

W Wu,G Mao,C Yu,

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[Changes of phosphorylation states of Cx43 protein in Chinese hamster lung cells induced by SiO2] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua lao dong wei sheng zhi ye bing za zhi = Z

VOLUME: 20

Page Numbers: 455-7

Journal Abbreviation: Zhonghua Lao Dong Wei Sheng Zh

ISSN: 1001-9391

DAY: 25

MONTH: Dec

YEAR: 2002

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LANGUAGE: chi

NlmUniqueID: 8410840

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AFFILIATION: Women's Hospital, Zhejiang University, School of Medicine, Hangzhou 310006, China.

Country: China

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MEDLINETA: Zhonghua Lao Dong Wei Sheng Zh

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