[Changes in water state and soluble protein contents of wampee axes during inducing desiccation tolerance by sucrose preculture]

[Changes in water state and soluble protein contents of wampee axes during inducing desiccation tolerance by sucrose preculture] Research Abstract Details 

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[Changes in water state and soluble protein contents of wampee axes during inducing desiccation tolerance by sucrose preculture] Abstract Text:

Xue-Mei Huang,Song-Quan Song,Jia-Rui Fu,

Progressively increasing sucrose concentration of culture medium could increase desiccation tolerance of wampee [Clausena lansium (Lour.) Skeels] axes. The changes in water state and soluble proteins of axes during acquirement of desiccation tolerance were measured by differential scanning calorimeter (DSC) and SDS-PAGE. The results showed that the cooling and heating thermograms of sucrose-precultured axes were similar to those of the control; but there was a stepwise change in the heating thermograms of sucrose-precultured axes, implying that vitrification might occur in axes. Unfreezable water amounts of wampee axes were measured, the results showed that amounts of unfreezable water of sucrose-precultured axes and control were 25.4% and 25.9% (by the linear regression equation method), or 24.3% and 23.7% (by the heat of ice fusion method) respectively, which were not significantly different. The soluble protein content of sucrose-precultured axes was 68% higher than the control, and SDS-PAGE showed that a 20-kD protein markedly increased in content.

[Changes in water state and soluble protein contents of wampee axes during inducing desiccation tolerance by sucrose preculture] Publishing Authors By Initials

XM Huang,SQ Song,JR Fu,

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[Changes in water state and soluble protein contents of wampee axes during inducing desiccation tolerance by sucrose preculture] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhi wu sheng li yu fen zi sheng wu xue xue bao = J

VOLUME: 32

Page Numbers: 245-51

Journal Abbreviation: Zhi Wu Sheng Li Yu Fen Zi Shen

ISSN: 1671-3877

DAY: 19

MONTH: Apr

YEAR: 2006

[Changes in water state and soluble protein contents of wampee axes during inducing desiccation tolerance by sucrose preculture] Information

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LANGUAGE: chi

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Grant and Affiliation Information for [Changes in water state and soluble protein contents of wampee axes during inducing desiccation tolerance by sucrose preculture]

AFFILIATION: College of Horticulture, South China Agricultural University, Guangzhou 510642, China. xmhuang@scau.edu.cn

Country: China

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MEDLINETA: Zhi Wu Sheng Li Yu Fen Zi Shen

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