Changes in the ratio between FPR and FPRL1 triggered superoxide production in human neutrophils-A tool in analysing receptor specific events.

Changes in the ratio between FPR and FPRL1 triggered superoxide production in human neutrophils-A tool in analysing receptor specific events. Research Abstract Details 

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Changes in the ratio between FPR and FPRL1 triggered superoxide production in human neutrophils-A tool in analysing receptor specific events. Abstract Text:

Huamei Fu,Jennie Karlsson,Lena Björkman,Anna-Lena Stenfeldt,Anna Karlsson,Johan Bylund,Claes Dahlgren,

Neutrophils express the G protein-coupled N-formyl peptide receptor (FPR) as well as its closely related homologue, formyl peptide like receptor 1 (FPRL1), and activation of these receptors induce a release of superoxide anions. The magnitude of the responses induced by the two peptide agonists fMLF and WKYMVM, specific for FPR and FPRL1, respectively, was found to be very variable in different neutrophil populations. The ratio between the FPR and FPRL1 triggered respiratory burst was, however, very constant and close to 1. The ratio was changed in neutrophils that were desensitized as well as when the signaling through either of the receptors was inhibited by receptor specific antagonists or by a PIP(2) binding peptide. The FPR/FPRL1 ratio was not changed in primed neutrophils or in differentiated HL-60 cells. We show that the change in the ratio, calculated from the amount of radical release in neutrophils triggered with FPR and FPRL1 specific agonists can be used as a valuable tool to find/identify receptor specific/selective changes mediated by peptides/proteins/drugs, as well as to identify cells from patients or groups of patients that diverge from normal cells in their FPR/FPRL1 triggered functions.

Changes in the ratio between FPR and FPRL1 triggered superoxide production in human neutrophils-A tool in analysing receptor specific events. Publishing Authors By Initials

H Fu,J Karlsson,L Björkman,AL Stenfeldt,A Karlsson,J Bylund,C Dahlgren,

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Changes in the ratio between FPR and FPRL1 triggered superoxide production in human neutrophils-A tool in analysing receptor specific events. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of immunological methods

VOLUME: 331

Page Numbers: 50-8

Journal Abbreviation: J. Immunol. Methods

ISSN: 0022-1759

DAY: 10

MONTH: 12

YEAR: 2007

Changes in the ratio between FPR and FPRL1 triggered superoxide production in human neutrophils-A tool in analysing receptor specific events. Information

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LANGUAGE: eng

NlmUniqueID: 1305440

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AFFILIATION: Department of Rheumatology and Inflammation Research, University of Göteborg, Guldhedsgatan 10, S-413 46 Göteborg, Sweden.

Country: Netherlands

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MEDLINETA: J Immunol Methods

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