Changes in the expression of cation-Cl- cotransporters, NKCC1 and KCC2, during cortical malformation induced by neonatal freeze-lesion.

Changes in the expression of cation-Cl- cotransporters, NKCC1 and KCC2, during cortical malformation induced by neonatal freeze-lesion. Research Abstract Details 

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Changes in the expression of cation-Cl- cotransporters, NKCC1 and KCC2, during cortical malformation induced by neonatal freeze-lesion. Abstract Text:

Chigusa Shimizu-Okabe,Akihito Okabe,Werner Kilb,Kohji Sato,Heiko J Luhmann,Atsuo Fukuda,Chigusa Shimizu-Okabe,Akihito Okabe,Werner Kilb,Kohji Sato,Heiko J Luhmann,Atsuo Fukuda,Chigusa Shimizu-Okabe,Akihito Okabe,Werner Kilb,Kohji Sato,Heiko J Luhmann,Atsuo Fukuda,

Focal cortical malformations comprise a heterogeneous group of disturbances in brain development, often associated with intractable epilepsy. A focal freeze-lesion of cerebral cortex in newborn rat produces a cortical malformation that resembles human polymicrogyria, clinical conditions that results from abnormal neuronal migration. The change in GABAergic functions that occurs during early brain development is induced by an alteration in Cl(-) homeostasis and plays important roles in neocortical development by modulating such events as laminar organization and synaptogenesis. We therefore investigated the relationship between pathogenesis of polymicrogyria and ontogeny of Cl(-) homeostasis in developing parietal cortex after creation of a freeze-lesion at P0. We demonstrated, by in situ hybridization histochemistry for cation-Cl(-) cotrtansporters, that NKCC1 mRNA expression was upregulated and KCC2 mRNA expression downregulated at P4 in "bridge" structure (formed in lesion site across the gap in intact exofocal cortex) as compared to exfocal cortex. Immunohistochemical investigation revealed a colocalization of NKCC1 and neuron specific enolase (NSE) within this structure, while BrdU-positive cells express GFAP and NKCC1 appeared beneath it. These results suggest that immature cortical plate neurons might produce "bridge" structure during formation of microgyrus, and that altered neuronal Cl(-) homeostasis might be involved in neuronal migration disorder that ultimately results in cortical malformations.

Changes in the expression of cation-Cl- cotransporters, NKCC1 and KCC2, during cortical malformation induced by neonatal freeze-lesion. Publishing Authors By Initials

C Shimizu-Okabe,A Okabe,W Kilb,K Sato,HJ Luhmann,A Fukuda,C Shimizu-Okabe,A Okabe,W Kilb,K Sato,HJ Luhmann,A Fukuda,C Shimizu-Okabe,A Okabe,W Kilb,K Sato,HJ Luhmann,A Fukuda,

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Changes in the expression of cation-Cl- cotransporters, NKCC1 and KCC2, during cortical malformation induced by neonatal freeze-lesion. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Neuroscience research

VOLUME: 59

Page Numbers: 288-95

Journal Abbreviation: Neurosci. Res.

ISSN: 0168-0102

DAY: 13

MONTH: 08

YEAR: 2007

Changes in the expression of cation-Cl- cotransporters, NKCC1 and KCC2, during cortical malformation induced by neonatal freeze-lesion. Information

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LANGUAGE: eng

NlmUniqueID: 8500749

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AFFILIATION: Department of Physiology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan. shimizuc@kph.bunri-u.ac.jp

Country: Ireland

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MEDLINETA: Neurosci Res

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