[Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene]

[Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene] Abstract Text:

Gang Chen,Zhong Wang,Qiao-Quan Liu,Fei Xiong,Yun-Jie Gu,Guo-Jun Gu,

The amylose content of rice caryopsis is determined by Wx protein, one kind of granule-bound starch synthetase which is encoded by Wx gene. Different rice types and species have different levels of Wx gene expression and have different amylose contents in their caryopsis. Wuyunjing No.7 (2200), the japonica rice with an amylose content 17% and its transgenic rice lines with antisense Wx gene (2201 and 2203, with amylose contents 8.5% and 2% respectively), and Longtefu (LP03), the indica rice with a high amylose content (28%) and its transgenic rice with antisense Wx gene (A199, with an amylose content 9%) were used to investigate the effects of Wx protein content decrease on the activities of enzymes involved in starch synthesis and thereby starch accumulation. The results indicated that with the decrease in Wx protein, the amylose content in transgenic caryopsis was reduced accordingly, whereas the amylopectin content per caryopsis (mg/grain) was not affected, and made the total starch content in transgenic caryopsis markedly lower than their parents. With the development of caryopsis, the amylose/total starch ratio was not changed significantly in the two parent caryopses, LP03 and 2200, but it went down gradually in their transgenic caryopses. The amylose/total starch ratio in transgenic caryopses was very significantly lower than their parents in the same period. The activities of ADP-glucose pyrophosphorylase (ADPG-PPase), granule-bound starch synthase (GBSS), soluble starch synthase (SSS) and starch branching enzyme (SBE) rose rapidly in early periods of grain filling, and soon reached their maximum, then reduced quickly until the middle and later periods of grain filling. Compared with the parents, the GBSS activity in transgenic caryopsis was significantly lowered, and correlated with the amylose reduction. Besides, the maximum activity of GBSS appeared earlier, and the range of the activity was smaller. In transgenic caryopsis, the activities of ADPG-PPase and SSS were higher than their parents in early and middle periods of grain filling, while the SBE activity was higher than their parents in middle and late periods.

[Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene] Publishing Authors By Initials

G Chen,Z Wang,QQ Liu,F Xiong,YJ Gu,GJ Gu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhi wu sheng li yu fen zi sheng wu xue xue bao = J

VOLUME: 32

Page Numbers: 209-16

Journal Abbreviation: Zhi Wu Sheng Li Yu Fen Zi Shen

ISSN: 1671-3877

DAY: 19

MONTH: Apr

YEAR: 2006

[Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 101156321

[Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene]

AFFILIATION: Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Agricultural College, Yangzhou University, Yangzhou 225009, China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Zhi Wu Sheng Li Yu Fen Zi Shen

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene Related Publications

• Bone graft in the shape of human mandibular condyle reconstruction via seeding marrow-derived osteoblasts into porous coral in a nude mice model.
• [A discuss on risk factors of final height in 21-hydroxylase deficiency patients]
• [Assessment of sleep disorders in children with Tourette's syndrome]
• [Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene]
• [Changes in the pulmonary function of factory workers exposure to terephthalic acid]
• [Clinical evaluation of two types of NiTi rotary instruments in preparation of root canals]
• [Comparative research on attenuation characteristics of human bladder cancer tissue at different wavelengths of laser and their linearly polarized laser in vitro]
• [Construction and expression in E. coli of single-chain F v fragment of antihuman activated platelets monoclonal antibody]
• [Culture and identification of human skin fibroblasts.]
• [Detection of IFN-gamma level in single CD8+ T cell]
• [Differentially expressed genes in hepatocellular carcinoma of tree shrew induced by different factors]
• [Effects of sodium nitroprusside on mitochondrial function of rye and wheat root tip under aluminum stress]
• [Human anti-HCMV neutralizing Fab antibody generated by phage display library]
• [IR and Raman spectroscopic studies on the complexes of ytterbium with pyran derivatives]
• [Induction of anti-tumor immunity by HSP gp96-peptide complexes]
• [Molecular genetic analysis of congenital lipoid adrenal hyperplasia]
• [Optical properties of human normal bladder tissue at five different wavelengths of laser and their linearly polarized laser irradiation in vitro]
• [Optical properties of human normal small intestine tissue with theoretical model of optics about biological tissues at Ar+ laser and 532 nm laser and their linearly polarized laser irradiation in vitro]
• [Prenatal diagnosis of glycogen storage disease Ia by screening for hot spot mutations in combination with the 1176 nucleotide polymorphism linkage analysis]
• [Production and some properties of a beta-glucanase from Trichoderma sp. GXC]
• [Purification and application of viral capsid antigen of Epstein-Barr virus]
• [Quantitative determination of insulin in microemulsion preparations by RP-HPLC]
• [Radial artery for coronary artery bypass grafting: operative techniques and early results]
• [Relation between pathologic tumor response to preoperative radiotherapy and the prognosis in patients with esophageal carcinoma]
• [Reporter genes transfer into human squamous cell carcinoma of larynx factor receptor mediated non-viral GE7 polypeptide delivery system with a new epidermal growth]
• [Research progress on the effect of zinc on behavior]
• [Solid phase extraction and room temperature phosphorimetry coupling technique and its application]
• [Study on the Raman spectra of ZYA system]
• [The applications of SERS to labeled immunoassay]
• [Value of intra-abdominal pressure monitoring in the prevention and treatment of multiple organ dysfunction syndrome]